Annexin-V imaging for noninvasive detection of cardiac allograft rejection
Annexin-V imaging for noninvasive detection of cardiac allograft rejection
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DOI：
10.1038/nm1201-1347
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发表时间：
2001-12-01
期刊：
影响因子：
82.9
通讯作者：
Strauss, HW
中科院分区：
文献类型：
--
作者：
Narula, J;Acio, ER;Strauss, HW
心脏移植排斥反应的病理学特征是心肌细胞坏死和凋亡，与间质性！单核细胞浸润。这些成分中的任何一种都可以作为移植排斥反应的非侵入性检测的靶点。在凋亡性细胞死亡过程中，磷脂酰丝氨酸（一种通常局限于细胞膜双层内叶的磷脂）被外置。锝-99m标记的膜联蛋白-V是一种内源性蛋白质，与磷脂酰丝氨酸结合具有高亲和力，已被静脉注射用于非侵入性鉴定凋亡细胞死亡。在本研究的18名心脏移植受者中，13名患者的心肌摄取膜联蛋白为阴性，5名为阳性。后五个表现出至少中度移植排斥和半胱天冬酶-3染色，表明在他们的活检标本凋亡。这项研究揭示了临床的可行性和安全性的膜联蛋白-V成像的非侵入性检测移植排斥反应的靶细胞膜磷脂的变化，通常与细胞凋亡的过程。
Heart transplant rejection is characterized pathologically by myocyte necrosis and apoptosis associated with interstitial! mononuclear cell infiltration. Any one of these components: can be targeted for noninvasive detection of transplant rejection. During apoptotic cell death, phosphatidylserine, a phospholipid that is normally confined to the inner leaflet of cell membrane bilayer, gets exteriorized. Technetium-99m-labeled annexin-V, an endogenous protein that has high affinity for binding to phosphatidylserine, has been administered intravenously for noninvasive identification of apoptotic cell death. In the present study of 18 cardiac allograft recipients, 13 patients had negative and five had positive myocardial uptake of annexin. These latter five demonstrated at least moderate transplant rejection and caspase-3 staining, suggesting apoptosis in their biopsy specimens. This study reveals the clinical feasibility and safety of annexin-V imaging for noninvasive detection of transplant rejection by targeting cell membrane phospholipid alterations that are commonly associated with the process of apoptosis.