Formicamycins, antibacterial polyketides produced by Streptomyces formicae isolated from African Tetraponera plant-ants.
Formicamycins, antibacterial polyketides produced by Streptomyces formicae isolated from African Tetraponera plant-ants.
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DOI：
10.1039/c6sc04265a
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发表时间：
2017-04-01
期刊：
影响因子：
8.4
通讯作者：
Hutchings MI
中科院分区：
文献类型：
--
作者：
Qin Z;Munnoch JT;Devine R;Holmes NA;Seipke RF;Wilkinson KA;Wilkinson B;Hutchings MI
蚂蚁嫁接：来自植物-蚂蚁相关细菌的抗菌聚酮化合物。本文报道了一个新的链霉菌属，命名为S.从非洲真菌生长的植物蚂蚁Tetraponera penzigi中分离的蚁科真菌，并显示其产生对MRSA和VRE有活性的新型五环聚酮化合物。这些化合物的化学支架，我们称之为甲酸霉素，与从环境DNA分离的克隆的异源表达中鉴定的法那霉素相似，但具有显著差异，允许支架用多达四个卤素原子装饰。我们报告了16种新分子的结构和生物活性，并使用CRISPR/Cas9基因组编辑显示，这些化合物的生物合成由S.蚁科基因组我们的工作已经从Tetraponera系统中鉴定出第一种抗生素，并强调了探索新放线菌菌株和新型天然产物的不寻常生态位的好处。
Ant pharming: antibacterial polyketides from plant-ant associated bacteria. We report a new Streptomyces species named S. formicae that was isolated from the African fungus-growing plant-ant Tetraponera penzigi and show that it produces novel pentacyclic polyketides that are active against MRSA and VRE. The chemical scaffold of these compounds, which we have called the formicamycins, is similar to the fasamycins identified from the heterologous expression of clones isolated from environmental DNA, but has significant differences that allow the scaffold to be decorated with up to four halogen atoms. We report the structures and bioactivities of 16 new molecules and show, using CRISPR/Cas9 genome editing, that biosynthesis of these compounds is encoded by a single type 2 polyketide synthase biosynthetic gene cluster in the S. formicae genome. Our work has identified the first antibiotic from the Tetraponera system and highlights the benefits of exploring unusual ecological niches for new actinomycete strains and novel natural products.