Assessment of Movement-Evoked Pain in Osteoarthritis by the Knee-Bend and CatWalk Tests: A Clinically Relevant Study
Assessment of Movement-Evoked Pain in Osteoarthritis by the Knee-Bend and CatWalk Tests: A Clinically Relevant Study
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DOI：
10.1016/j.jpain.2008.05.012
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发表时间：
2008-10-01
期刊：
影响因子：
4
通讯作者：
Castro-Lopes, Jose M.
中科院分区：
文献类型：
--
作者：
Ferreira-Gomes, Joana;Adaes, Sara;Castro-Lopes, Jose M.
虽然有一些关于膝骨关节炎（OA）大鼠模型疼痛行为测试的报道，但大多数集中在爪子上。本研究的目的是研究疼痛相关的行为对受影响的膝关节，伤害性感受的主要来源，在动物与单碘乙酸诱导的OA，使用膝盖弯曲（提供有关运动疼痛的信息）和针刺试验，并评估伤害性感受引起的步行使用猫走试验。应用于爪子的von Frey和Randall-Selitto测试使我们能够将我们的研究结果与以前的研究进行比较。另一个目的是比较最常用剂量的单碘乙酸盐（2和3 mg）的行为伤害性反应。在整个研究过程中，OA动物的膝关节弯曲评分高于对照动物（P <0.05）。猫走实验显示，OA大鼠在各时间点的后爪负荷均低于对照组（P <0.05），缩足阈值也低于对照组（P <0.01）。在针刺和Randall-Selitto试验中未观察到变化。2种剂量的单碘乙酸盐获得的结果相似。膝盖弯曲和猫步测试是有效的运动相关的伤害性感受，临床CIA的标志，这是目前整个实验period.Perspective：行为表征的模型OA疼痛是重要的和有用的，用于在未来的研究，以测试药物治疗。此外，重要的是找到与OA的人类症状更好地相关的方法。(C)2008年美国疼痛协会
Although there are several reports on pain behavioral tests in rat models of knee osteoarthritis (OA), most of them focus on the paw. The aim of this study was to investigate pain-related behaviors on the affected knee joint, the primary source of nociception, in animals with mono-iodoacetate-induced OA, using the knee-bend (which provides information on movement pain) and pin-prick tests, and to evaluate nociception elicited by walking using the CatWalk test. The von Frey and Randall-Selitto tests applied to the paw allowed us to compare our study results with previous studies. A further aim was to compare the behavioral nociceptive responses of the most used doses of mono-iodoacetate, 2 and 3 mg. Knee-bend score of OA animals was higher than those of control animals throughout the study (P < .05). At every time point, the ipsilateral hind-paw load of OA rats, as measured by the CatWalk test, was lower than that of control rats (P < .05), and paw withdraw threshold to von Frey filaments was also decreased (P < .01). No changes were observed in pin-prick and Randall-Selitto tests. Results obtained with the 2 doses of mono-iodoacetate were similar. The knee-bend and CatWalk tests are effective for evaluating movement-related nociception, a hallmark of clinical CIA, which was present throughout the experimental period.Perspective: Behavioral characterization of models of OA pain is important and useful for use in future studies to test pharmacological treatments. Furthermore, it is important to find methods that correlate better with the human symptoms of OA. (C) 2008 by the American Pain Society