Stray dogs in Nepal have high prevalence of vector-borne pathogens: a molecular survey
Stray dogs in Nepal have high prevalence of vector-borne pathogens: a molecular survey
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DOI：
10.1186/s13071-020-04057-7
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发表时间：
2020-04-21
影响因子：
3.2
通讯作者：
Sainz, Angel
中科院分区：
文献类型：
--
作者：
Diaz-Reganon, David;Agulla, Beatriz;Sainz, Angel
背景在尼泊尔的大城市，如加德满都，流浪狗的数量很大。大多数流浪狗缺乏基本的医疗保健。考虑到尼泊尔犬病媒传播疾病（CVBD）的临床相关性、广泛分布和信息缺乏，本研究的目的是评估生活在加德满都大都市区的流浪犬中不同病媒传播病原体（VBP）的患病率，方法在加德满都动物治疗中心采集70只流浪犬的血液样本，2017年8月期间的中心收集在滤纸上（弗林德斯技术协会（FTA）卡）。记录每只动物的体征、临床体征和流行病学特征数据。对利什曼原虫属进行实时聚合酶链反应测定，埃里希体属/无形体属，巴贝虫属/泰勒虫结果共检出犬肝体31.43%，扁平无形体31.43%，犬埃立克体27.14%，杜氏利什曼原虫18.57%，泰勒虫12.86%，福氏巴氏杆菌为12.86%，B为2.86%。吉布森尼。共有81.43%的狗对至少一种检测的VBP呈阳性。混合感染率为41.43%。对任何一种VBP测试呈阳性的狗，特别是对E。结论这是首次从尼泊尔加德满都的流浪狗中检测到VBP。由于越南船民个案的高发病率，我们有需要在该区的流浪狗群中推行监察计划和控制策略。
BackgroundPopulation of stray dogs is significant in large cities of Nepal, such as Kathmandu. Most of stray dogs suffer a lack of basic health care. Considering the clinical relevance, the broad distribution and the lack of information of canine vector borne diseases (CVBD) in Nepal, the aim of this study was to evaluate the prevalence of different vector-borne pathogens (VBP) in stray dogs living in the metropolitan area of Kathmandu, and to assess different traits as possible risk factors.MethodsA total of 70 canine blood samples from stray dogs attended at the Kathmandu Animal Treatment Centre during August 2017 were collected on filter paper (Flinders Technology Associates (FTA) cards). Data regarding signalment, clinical signs and epidemiological characteristics were recorded for each animal. Real-time polymerase chain reaction assays were performed for Leishmania spp., Ehrlichia spp./Anaplasma spp., Babesia spp./Theileria spp. and Hepatozoon canis.ResultsThe overall prevalence detected was 31.43% for Hepatozoon canis, 31.43% for Anaplasma platys, 27.14% for Ehrlichia canis, 18.57% for Leishmania donovani species complex, 12.86% for isolates corresponding to Theileria spp., 12.86% for Babesia vogeli and 2.86% for B. gibsoni. A total of 81.43% of the dogs were positive to at least one of the VBP tested. Co-infections were detected in 41.43% of the dogs. Dogs positive to any of the VBP tested, and particularly to E. canis, were older than those that were negative.ConclusionsTo our knowledge, this is the first molecular detection of VBP in stray dogs from Kathmandu, Nepal. The high prevalence of VBP detected highlights the need to implement a surveillance programme and control strategies for these CVBD in the population of stray dogs in this area.