SOME CHARACTERISTICS OF HEMOLYSIS BY ULTRAVIOLET LIGHT
SOME CHARACTERISTICS OF HEMOLYSIS BY ULTRAVIOLET LIGHT
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DOI：
10.1002/jcp.1030470105
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发表时间：
1956-01-01
影响因子：
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通讯作者：
COOK, JS
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文献类型：
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作者：
COOK, JS
通过显微照相视野计数研究了短暂暴露于紫外线辐射后哺乳动物红细胞的溶血情况。紫外线溶血是一种“全有或全无”的现象，因为溶血时，每个红细胞都会释放其所有血红蛋白，以达到与环境的扩散平衡。在校正细胞对渗透压的阻力分布后，溶血速率似乎是时间的线性函数。溶血率与入射辐射强度无关，即遵循互易定律，并且与辐射总剂量的平方成正比。该指数与研究范围（5°C 至 37°C）内的温度无关。在所采用的条件下，反应一旦引发，就是不可逆的。波长为0.1850μ的辐射。比波长为0.2537μ的辐射更有效。紫外线溶血的 Q10 约为 2。溶血在渗透压降低的溶液中加速，并被外部环境中蔗糖的存在所抑制。这些因素被解释为表明紫外线溶血本质上是胶体渗透性的。在 pH 值范围为 5.8 至 7.8 的溶液中，随着 pH 值的增加，紫外线溶血会加速。这种行为起初似乎与胶体渗透溶血理论不一致，但被解释为细胞膜中初始光化学反应的特征。与某些其他光化学反应类比，波长数据和pH数据与光吸收剂本质上是蛋白质的假设一致。
Hemolysis of mammalian red blood cells following brief exposure to ultraviolet radiation has been studied by counts of microphotographic fields. Ultraviolet hemolysis is an "all-or-none" phenomenon in that, upon hemolysis, each red cell releases all of its hemoglobin to diffusion equilibrium with the environment After correction for the distribution of the cells'' resistances to osmotic pressure, the rate of hemolysis appears to be a linear function of time. The rate of hemolysis is independent of the intensity of the incident radiation, i.e., the Reciprocity Law is obeyed, and is proportional to the square of the total dose of radiation. This exponent is independent of the temperature over the range studied (5[degree] to 37[degree]C). Under the conditions employed, the reaction, once initiated, is irreversible. Radiation of wavelength .1850 [mu]. is of much greater effectiveness than radiation of wavelength .2537 [mu]. The Q10 for ultraviolet hemolysis is approximately 2. Hemolysis is accelerated in solutions of decreased osmotic pressure and is inhibited by the presence of sucrose in the external environment. These factors are interpreted as indicating that ultraviolet hemolysis is colloid osmotic in nature. Over the pH range 5.8 to 7.8 ultraviolet hemolysis is accelerated in solutions of increasing pH. This behavior, which at first appears inconsistent with the theory of colloid osmotic hemolysis, is interpreted as a characteristic of the initial photochemical reaction in the cell membrane. By analogy with certain other photochemical reactions, the wavelength data and pH data are consistent with the hypothesis that the light absorber is protein in nature.