Growth hormone regulation of rat liver gene expression assessed by SSH and microarray
Growth hormone regulation of rat liver gene expression assessed by SSH and microarray
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DOI：
10.1016/s0303-7207(02)00004-7
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发表时间：
2002-04-25
影响因子：
4.1
通讯作者：
Mode, A
中科院分区：
文献类型：
--
作者：
Gardmo, C;Swerdlow, H;Mode, A
生长激素（GH）的两性二态分泌在大鼠中普遍存在，导致GH靶基因的性别分化表达，特别是在肝脏中。我们使用减法抑制杂交（SSH）来寻找由雌性特征的、接近连续的生长激素分泌模式诱导的新的靶基因。利用微阵列和斑点杂交技术，试图确定获得的SSH克隆的差异表达率。在173个唯一的SSH克隆中，有41个可以验证为差异表达。在这些基因中，我们发现了17个已知的基因，这些基因以前没有被认为是由性别特异性生长激素模式差异调节的。其他SSH克隆也可能代表受性别特异性生长激素调控的基因，因为只有大量表达的转录本才能得到验证。优化分析，具体到每个基因，需要充分表征差异表达的程度。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
The sexually dimorphic secretion of growth hormone (GH) that prevails in the rat leads to a sex-differentiated expression of GH target genes, particularly in the liver. We have used subtractive Suppressive hybridization (SSH) to search for new target genes induced by the female-characteristic, near continuous, pattern of GH secretion. Microarrays and dot-blot hybridizations were used in an attempt to confirm differential ratios of expression of obtained SSH clones. Out of 173 unique SSH clones, 41 could be verified as differentially expressed. Among these, we identified 17 known genes not previously recognized as differentially regulated by the sex-specific GH pattern. Additional SSH clones may also represent genes subjected to sex-specific GH regulation since only transcripts abundantly expressed could be verified. Optimized analyses, specific for each gene, are required to fully characterize the degree of differential expression. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.