TISSUE-SPECIFIC METABOLISM DURING NORMOTHERMY AND DAILY TORPOR IN DEER MICE (PEROMYSCUS-MANICULATUS)
TISSUE-SPECIFIC METABOLISM DURING NORMOTHERMY AND DAILY TORPOR IN DEER MICE (PEROMYSCUS-MANICULATUS)
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DOI：
10.1002/jez.1402610407
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发表时间：
1992-04-01
影响因子：
--
通讯作者：
NESTLER, JR
中科院分区：
文献类型：
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作者：
NESTLER, JR
先前对鹿鼠（Peromyscus maniculatus）的研究表明，日常麻木期间会发生明显的酸中毒。此外，在休眠期间，碳水化合物水平显着降低，而脂肪酸和酮水平显着升高。本研究研究了这些体内酸碱和代谢物调整对取自正常体温和迟钝鹿小鼠的组织的体外 C-14-葡萄糖代谢的影响。取自常温动物的肝脏和股外侧肌中的葡萄糖氧化通过将培养温度从 37 摄氏度更改为 25 摄氏度而减少（肝脏，0.44 至 0.23 微摩尔/克小时；股肌，0.66 至 0.25 微摩尔/克小时），而来自常温小鼠的心脏则表现出从 0.57 增加到较低温度下为 0.99-mu-moles/g.h。改变酸碱条件或代谢物水平对心脏或肝脏的葡萄糖代谢没有影响。然而，这两个因素都显着影响股骨头的新陈代谢。取自常温小鼠的 Vastus 在更酸性的休眠条件下（0.25 至 0.33-mu-moles/g-h）具有增加的葡萄糖氧化速率，而当与休眠底物浓度（0.33 至 0.22-mu-moles/g-h）一起孵育时，氧化发生减少。在取自麻木小鼠的股骨中，将酸碱状态和代谢物改变至麻木水平导致葡萄糖氧化显着减少（0.26至0.12-mu-moles/g-h）。在大多数孵化条件下，取自麻木小鼠的组织中的葡萄糖氧化明显低于取自常温小鼠的组织，这表明除了酸碱和底物参数的变化之外，可能还需要进行调整以解释日常麻木期间的代谢变化。
Previous work with deer mice (Peromyscus maniculatus) has demonstrated that a significant acidosis occurs during daily torpor. In addition, carbohydrate levels are significantly lower, whereas fatty acid and ketone levels are significantly higher during torpor. The present stud examined the effects of these in vivo acid-base and metabolite adjustments on in vitro C-14-glucose metabolism in tissues taken from normothermic and torpid deer mice. Glucose oxidation in liver and vastus lateralis taken from normothermic animals was reduced by a change in incubation temperature from 37-degrees-C to 25-degrees-C (liver, 0.44 to 0.23-mu-moles/g.h; vastus, 0.66 to 0.25-mu-moles/g.h), whereas heart from normothermic mice exhibited an increase from 0.57 to 0.99-mu-moles/g.h at the lower temperature. Altering acid-base conditions or metabolite levels had no effect on glucose metabolism in the heart or liver. However, both of these factors significantly influenced metabolism in vastus. Vastus taken from normothermic mice had an increased glucose oxidation rate under the more acidic torpor conditions (0.25 to 0.33-mu-moles/g-h), whereas a reduction in oxidation occurred when incubated with torpor substrate concentrations (0.33 to 0.22-mu-moles/g-h). In vastus taken from torpid mice, changing acid-base state and metabolites to torpor levels resulted in a significant reduction in glucose oxidation (0.26 to 0.12-mu-moles/g-h). Under most incubation conditions, glucose oxidation was significantly lower in tissues taken from torpid mice than in these tissues from normothermic mice, suggesting that adjustments in addition to changes in acid-base and substrate parameters may be necessary to account for metabolic modifications during daily torpor.