Agonists of PPAR-α, PPAR-γ, and RXR inhibit the formation of foam cells from macrophages in mice with inflammation
Agonists of PPAR-α, PPAR-γ, and RXR inhibit the formation of foam cells from macrophages in mice with inflammation
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DOI：
10.1007/s10517-007-0413-3
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发表时间：
2007-11-01
影响因子：
0.7
通讯作者：
Schvarts, Y. Sh.
中科院分区：
文献类型：
--
作者：
Dushkin, M. I.;Khoshchenko, O. M.;Schvarts, Y. Sh.
本文研究了过氧化体增殖物激活受体α、γ受体激动剂和维甲酸X受体激动剂对C57B1/6小鼠腹腔注射酵母多糖所致炎症模型小鼠巨噬细胞脂质浓度和合成的影响。结果表明，酵母多糖50 mg/kg给药24 h后，小鼠腹腔巨噬细胞[1-C-14]油酸向胆固醇酯和甘油三酯的掺入显著增加，游离胆固醇、胆固醇酯和甘油三酯的含量增加，并形成油红色脂质包涵体。在酵母多糖注射前30min和注射后12h分别给予维甲酸X受体激动剂和过氧化物酶体增殖物激活受体α和γ处理，可减少巨噬细胞内甘油三酯和胆固醇酯的合成，降低巨噬细胞内游离胆固醇、胆固醇酯和甘油三酯的含量，并防止巨噬细胞源性泡沫细胞在炎症过程中形成细胞质脂质包涵体。
We studied the effect of agonists of peroxisome proliferator-activated receptors alpha and gamma and retinoid X receptors on the concentration and synthesis of lipids in macrophages of C57B1/6 mice with inflammation induced by intraperitoneal injection of zymosan. We revealed a significant increase in [1-C-14]oleate incorporation into cholesterol esters and triglycerides, increase in the content of free cholesterol, cholesterol esters, and triglycerides, and formation of oil red-stained lipid inclusions in peritoneal macrophages 24 h after administration of zymosan in a dose of 50 mg/kg. Treatment with agonists of retinoid X receptors and peroxisome proliferator-activated receptors alpha and gamma 30 min before and 12 h after zymosan injection decreased the synthesis of triglycerides and cholesterol esters, reduced the content of free cholesterol, cholesterol esters, and triglycerides in macrophages, and prevented the formation of cytoplasmic lipid inclusions in macrophage-derived foam cells during inflammation.