HUMAN CELLULAR FIBRONECTIN - COMPARISON OF THE CARBOXYL-TERMINAL PORTION WITH RAT IDENTIFIES PRIMARY STRUCTURAL DOMAINS SEPARATED BY HYPERVARIABLE REGIONS
HUMAN CELLULAR FIBRONECTIN - COMPARISON OF THE CARBOXYL-TERMINAL PORTION WITH RAT IDENTIFIES PRIMARY STRUCTURAL DOMAINS SEPARATED BY HYPERVARIABLE REGIONS
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DOI：
10.1021/bi00332a016
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
CHU, ML
中科院分区：
文献类型：
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作者：
BERNARD, MP;KOLBE, M;CHU, ML
分离和鉴定编码人细胞纤连蛋白的4个重叠cDNA克隆，其长度连续覆盖> 3个酶。这些cDNA的核苷酸序列进行了测定，从而阐明了人纤连蛋白的C-末端794个残基的氨基酸序列，其覆盖了该蛋白的细胞，肝素和纤维蛋白结合结构域的边缘。核苷酸序列和推导的氨基酸序列与大鼠的核苷酸序列和推导的氨基酸序列的比较[Schwarzbauer，J.E.，Tamkun，J.W.，列米施卡，IR，和Hynes，R.O.（1983）]表明在核苷酸和氨基酸水平上的高度保守性。与以前发表的牛纤连蛋白的氨基酸序列的数据进行比较，有可能确定在人类，小牛和大鼠的进化过程中的蛋白质的结构上的重要特征。推导的人氨基酸序列含有5个III型和3个I型重复的内部同源性。氨基酸的种间保守性在含有内部重复序列的区域和每个功能结构域内更为明显。这些物种间的保护和分歧的影响进行了讨论。
The isolation and characterization of 4 overlapping cDNA clones coding for human cellular fibronectin which continuously cover > 3 kilobases in length. The nucleotide sequence of these cDNA was determined, thus elucidating the amino acid sequence of the C-terminal 794 residues of human fibronectin, which cover the edge of cellular-, heparin- and fibrin-binding domains of this protein. Comparisons of the nucleotide sequences and the deduced amino acid sequences with those of rat [Schwarzbauer, J.E., Tamkun, J.W., Lemischka, I.R., and Hynes, R.O. (1983)] indicate a high degree of conservation at both nucleotide and amino acid levels. Comparison with previously published data on amino acid sequences of bovine fibronectin made it possible to identify structurally important features of the protein during the evolution of human, calf and rat. The deduced human amino acid sequences contain 5 type III and 3 type I repeats of internal homologies. The interspecies conservation in amino acids is more pronounced in regions containing the internal repeats and within each functional domain. The implications of these interspecies conservation and divergence are discussed.