Immunization with A2 protein results in a mixed Th1/Th2 and a humoral response which protects mice against Leishmania donovani infections
Immunization with A2 protein results in a mixed Th1/Th2 and a humoral response which protects mice against Leishmania donovani infections
复制标题
DOI：
10.1016/s0264-410x(01)00322-x
复制
发表时间：
2001-10-12
期刊：
影响因子：
5.5
通讯作者：
Matlashewski, G
中科院分区：
文献类型：
--
作者：
Ghosh, A;Zhang, WW;Matlashewski, G
多诺瓦利什曼原虫的A2基因编码无马鞭毛体特异性A2蛋白，该蛋白被认为是这种原生动物寄生虫在哺乳动物宿主中存活所需的毒力因子。A2基因存在于一个多基因家族中，相应的A2蛋白主要由10个氨基酸重复序列的多个拷贝组成。在内脏利什曼病（VL）患者的血清中检测到A2特异性抗体，并表明产生A2缺陷的L. donovani导致无毒表型。在本报告中，我们发现重组A2蛋白免疫小鼠对多诺瓦氏乳杆菌的攻击感染具有显著的保护作用。这种保护作用与体外脾细胞增殖、响应A2蛋白产生ifn - γ以及免疫小鼠血清中A2特异性抗体的存在有关。这些数据表明，A2是一种潜在的抗原，可以保护机体免受多诺瓦氏乳杆菌和VL的感染。(C) 2001年Elsevier Science Ltd.出版
The A2 genes of Leishmania donovani encode amastigote-specific A2 proteins, which are considered to be virulence factors required for the survival of this protozoan parasite in the mammalian host. The A2 genes are present within a multigene family and corresponding A2 proteins are composed predominantly of multiple copies of a 10 amino acid repeat sequences. A2-specific antibodies have been detected in the sera of patients suffering from visceral leishmaniasis (VL) and it has been shown that generation of A2 deficient L. donovani resulted in an avirulent phenotype. In this report, we show that immunization of mice with recombinant A2 protein conferred significant protection against challenge infection with L. donovani. The protection correlated with in vitro splenocyte proliferation, production of IFN-gamma in response to A2 protein and the presence of A2-specific antibodies in the sera of immunized mice. These data demonstrate that A2 represents a potential antigen for protection against infection with L. donovani and VL. (C) 2001 Published by Elsevier Science Ltd.