Widespread Inducible Transcription Downstream of Human Genes.
Widespread Inducible Transcription Downstream of Human Genes.
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DOI：
10.1016/j.molcel.2015.06.016
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发表时间：
2015-08-06
期刊：
影响因子：
16
通讯作者：
Steitz JA
中科院分区：
文献类型：
--
作者：
Vilborg A;Passarelli MC;Yario TA;Tycowski KT;Steitz JA
人类基因组的普遍转录产生RNA，其形成和功能的模式在很大程度上是未知的。在这里，我们将联合收割机RNA-Seq与详细的机制研究相结合，以描述来自蛋白质编码基因的转录本类型。所得到的RNA，我们称之为含有转录物的基因下游的DoG，具有长的非编码区（通常>45 kb）并保持染色质结合。通过IP 3受体信号传导依赖性途径，渗透胁迫可诱导DoG，表明其具有主动调节作用。上游转录物的终止减少增加了DoG水平，这是一种以前未描述的快速转录物诱导机制。多聚腺苷酸信号在DoG区域的相对消耗与渗透胁迫后DoG水平的增加相关。我们在几种人类细胞系中检测到DoG转录，并为数千个全基因组的DoG提供证据。
Pervasive transcription of the human genome generates RNAs whose mode of formation and functions are largely uncharacterized. Here, we combine RNA-Seq with detailed mechanistic studies to describe a transcript type derived from protein-coding genes. The resulting RNAs, which we call DoGs for downstream of gene containing transcripts, possess long non-coding regions (often >45 kb) and remain chromatin bound. DoGs are inducible by osmotic stress through an IP3 receptor signaling-dependent pathway, indicating active regulation. DoG levels are increased by decreased termination of the upstream transcript, a previously undescribed mechanism for rapid transcript induction. Relative depletion of polyA signals in DoG regions correlates with increased levels of DoGs after osmotic stress. We detect DoG transcription in several human cell lines and provide evidence for thousands of DoGs genome-wide.