Identification of enterovirus 71 isolates from an outbreak of hand, foot and mouth disease (HFMD) with fatal cases of encephalomyelitis in Malaysia
Identification of enterovirus 71 isolates from an outbreak of hand, foot and mouth disease (HFMD) with fatal cases of encephalomyelitis in Malaysia
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DOI：
10.1016/s0168-1702(99)00019-2
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发表时间：
1999-05-01
期刊：
影响因子：
5
通讯作者：
Lam, SK
中科院分区：
文献类型：
--
作者：
AbuBakar, S;Chee, HY;Lam, SK
在1997年马来西亚爆发手足口病期间，从马来西亚半岛和沙捞越患者的致命性和非致命性感染中获得了13株肠道病毒71型(EV71)，并发生了致命性脑干脑脊髓炎。用免疫荧光染色、中和试验和5‘非翻译区(UTR)部分测序对分离株进行鉴定。使用部分5‘非编码区序列对分离株的潜在遗传关系的评估表明，分离株至少聚为两个主要簇。来自马来西亚半岛的分离物在两个聚类群中都被发现，而来自沙捞越的分离物只在聚类群II中被发现。然而，来自致命性感染的分离物在两个聚类物中都存在，并且没有明显的核苷酸序列可以归因于致命性分离物。对核苷酸序列的检测显示，所有分离株中至少有13个核苷酸位置，与以前报道的EV71 5‘UTR序列完全不同。此外，在区分簇I和簇II的5‘非编码区内至少有11个核苷酸位置的差异。使用核苷酸序列绘制的预测二级RNA结构也表明来自这两个簇的分离物之间的差异。这些发现表明，在1997年手足口病暴发期间，马来西亚至少存在两种共同传播的潜在毒力EV71型病毒。(C)1999 Elsevier Science B.V.保留所有权利。
Thirteen enterovirus 71 (EV71) isolates were obtained from both fatal and non-fatal infections of patients seen in Peninsula Malaysia and in Sarawak during an outbreak of hand, foot and mouth disease (HFMD) in Malaysia in 1997, with incidences of fatal brainstem encephalomyelitis. The isolates were identified using immunofluorescence staining, neutralization assays, and partial sequencing of the 5' untranslated regions (UTR). Assessment of the potential genetic relationships of the isolates using the partial 5'UTR sequences suggested clustering of the isolates into at least two main clusters. Isolates from Peninsula Malaysia were found in both clusters whereas Sarawak-derived isolates clustered only in cluster II. Isolates derived from fatal infections, however, occurred in both clusters and no distinctive nucleotide sequences could be attributed to the fatal isolates. Examination of the nucleotide sequences revealed at least 13 nucleotide positions in all the isolates which differ completely from the previously reported EV71 5'UTR sequences. In addition, at least 11 nucleotide position differences within the 5'UTR were noted which differentiated cluster I from cluster II. Predicted secondary RNA structures drawn using the nucleotide sequences also suggested differences between isolates from the two clusters. These findings suggest the presence of at least two potentially virulent EV71 co-circulating in Malaysia during the 1997 HFMD outbreak. (C) 1999 Elsevier Science B.V. All rights reserved.