Combined ingestion of protein and free leucine with carbohydrate increases postexercise muscle protein synthesis in vivo in male subjects
Combined ingestion of protein and free leucine with carbohydrate increases postexercise muscle protein synthesis in vivo in male subjects
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DOI：
10.1152/ajpendo.00413.2004
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发表时间：
2005-04-01
影响因子：
5.1
通讯作者：
van Loon, LJC
中科院分区：
文献类型：
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作者：
Koopman, R;Wagenmakers, AJM;van Loon, LJC
本研究旨在确定运动后肌肉蛋白质合成和全身蛋白质平衡后，结合摄入碳水化合物与或不与蛋白质和/或游离亮氨酸。八名男性受试者被随机分配到三个试验中，他们在45分钟的抗阻运动后饮用含有碳水化合物（CHO），碳水化合物和蛋白质（CHO+PRO）或碳水化合物，蛋白质和游离亮氨酸（CHO+PRO+Leu）的饮料。应用L-[环-C-13（6）]苯丙氨酸的预充、连续输注，采集血液样本和肌肉活检，以评估股外侧肌中的分数合成率（FSR）以及运动后恢复6小时期间的全身蛋白质周转。与CHO和CHO+PRO试验相比，CHO+PRO+Leu中的血浆胰岛素反应更高（分别为+240 +/- 19%和+77 +/-11%，P < 0.05）。与CHO试验相比，CHO+PRO和CHO+PRO+Leu试验的整体蛋白质分解率较低，整体蛋白质合成率较高（P < 0.05）。与CHO+PRO试验相比，在CHO+PRO+Leu试验中添加亮氨酸导致较低的蛋白质氧化速率。在CHO试验中，蛋白质平衡在恢复期间为阴性，但在CHO+PRO和CHO+PRO+Leu试验中为阳性。在CHO+PRO+Leu试验中，与CHO+PRO和CHO试验中观察到的值相比，全身净蛋白平衡显著更大（P < 0.05）。在6小时运动后恢复期内测量的混合肌肉FSR，在CHO+PRO+Leu试验中显著高于CHO试验（分别为0.095 +/- 0.006 vs. 0.061 +/- 0.008%/h，P < 0.05），在CHO+PRO试验中观察到中间值（0.0820 +/- 0.0104%/h）。我们的结论是，与只摄入碳水化合物相比，蛋白质和亮氨酸的共同摄入刺激肌肉蛋白质合成，优化全身蛋白质平衡。
The present study was designed to determine postexercise muscle protein synthesis and whole body protein balance following the combined ingestion of carbohydrate with or without protein and/or free leucine. Eight male subjects were randomly assigned to three trials in which they consumed drinks containing either carbohydrate (CHO), carbohydrate and protein (CHO+PRO), or carbohydrate, protein, and free leucine (CHO+PRO+Leu) following 45 min of resistance exercise. A primed, continuous infusion of L-[ring-C-13(6)] phenylalanine was applied, with blood samples and muscle biopsies collected to assess fractional synthetic rate (FSR) in the vastus lateralis muscle as well as whole body protein turnover during 6 h of postexercise recovery. Plasma insulin response was higher in the CHO+PRO+Leu compared with the CHO and CHO+PRO trials (+240 +/- 19% and +77 +/- 11%, respectively, P < 0.05). Whole body protein breakdown rates were lower, and whole body protein synthesis rates were higher, in the CHO+PRO and CHO+PRO+Leu trials compared with the CHO trial (P < 0.05). Addition of leucine in the CHO+PRO+Leu trial resulted in a lower protein oxidation rate compared with the CHO+PRO trial. Protein balance was negative during recovery in the CHO trial but positive in the CHO+PRO and CHO+PRO+Leu trials. In the CHO+PRO+Leu trial, whole body net protein balance was significantly greater compared with values observed in the CHO+PRO and CHO trials (P < 0.05). Mixed muscle FSR, measured over a 6-h period of postexercise recovery, was significantly greater in the CHO+PRO+Leu trial compared with the CHO trial (0.095 +/- 0.006 vs. 0.061 +/- 0.008%/h, respectively, P < 0.05), with intermediate values observed in the CHO+PRO trial (0.0820 +/- 0.0104%/h). We conclude that coingestion of protein and leucine stimulates muscle protein synthesis and optimizes whole body protein balance compared with the intake of carbohydrate only.