MicroRNA-137 promoter methylation is associated with poorer overall survival in patients with squamous cell carcinoma of the head and neck.
MicroRNA-137 promoter methylation is associated with poorer overall survival in patients with squamous cell carcinoma of the head and neck.
复制标题
DOI：
10.1002/cncr.25689
复制
发表时间：
2011-04-01
期刊：
影响因子：
6.2
通讯作者：
Taioli, Emanuela
中科院分区：
文献类型：
--
作者：
Langevin, Scott M.;Stone, Roslyn A.;Bunker, Clareann H.;Lyons-Weiler, Maureen A.;LaFramboise, William A.;Kelly, Lori;Seethala, Raja R.;Grandis, Jennifer R.;Sobol, Robert W.;Taioli, Emanuela
在过去的30年中，头颈癌患者的总体5年生存率约为60%，基本保持不变。microRNA-137（miR-137）在细胞周期控制中在G1/S期检查点起重要作用。然而，在头颈部鳞状细胞癌（SCCHN）中观察到的异常miR-137启动子甲基化表明可能导致疾病进展的肿瘤特异性分子缺陷。本研究的目的是在福尔马林固定的石蜡包埋的肿瘤组织中评估miR-137启动子甲基化与生存（总体和无病）以及预后因素（包括分期、肿瘤大小、淋巴结阳性、肿瘤分级和手术肿瘤切缘阳性）之间的相关性。通过甲基化特异性PCR确定miR-137的启动子甲基化状态，并在11/67例SCCHN患者（16.4%）中检测到，根据部位（口腔，咽，喉）无显著差异。miR-137启动子的甲基化与总生存率显著相关（风险比= 3.68，95%置信区间：1.01-13.38），但与无病生存率或任何评估的预后因素无关。这项研究表明，miR-137甲基化的肿瘤组织，从咽和喉鳞状细胞癌，除了口腔鳞状细胞癌;和miR-137启动子甲基化有潜在的效用作为SCCHN的预后标志物。
The overall 5-year survival rate of approximately 60% for head and neck cancer patients has remained essentially unchanged over the past 30 years. MicroRNA-137 (miR-137) plays an essential role in cell cycle control at the G1/S phase checkpoint. However, aberrant miR-137 promoter methylation observed in squamous cell carcinoma of the head and neck (SCCHN) suggests a tumor-specific molecular defect that may contribute to disease progression. The goal of this study is to assess, in formalin-fixed paraffin-embedded tumor tissue, the association between miR-137 promoter methylation and survival (both overall and disease-free) and with prognostic factors including stage, tumor size, nodal positivity, tumor grade and surgical tumor margin positivity. Promoter methylation status of miR-137 was ascertained by methylation-specific PCR and detected in 11/67 SCCHN patients (16.4%), with no significant differences according to site (oral cavity, pharynx, larynx). Methylation of the miR-137 promoter was significantly associated with overall survival (Hazard Ratio = 3.68, 95% Confidence Interval: 1.01–13.38) but not with disease-free survival or any of the prognostic factors evaluated. This study indicates that miR-137 is methylated in tumor tissue from pharyngeal and laryngeal squamous cancers, in addition to oral squamous cell carcinoma; and that miR-137 promoter methylation has potential utility as a prognostic marker for SCCHN.
登录
查看更多内容
影响因子：
6.4
作者：
Chang, Steven S.;Jiang, Wei Wen;Smith, Ian;Poeta, Luana M.;Begum, Shahnaz;Glazer, Chad;Shan, Shannon;Westra, William;Sidransky, David;Califano, Joseph A.
通讯作者：
Califano, Joseph A.
影响因子：
11.2
作者：
Huang YW;Liu JC;Deatherage DE;Luo J;Mutch DG;Goodfellow PJ;Miller DS;Huang TH
通讯作者：
Huang TH
影响因子：
11.2
作者：
Kozaki, Ken-ichi;Imoto, Issei;Inazawa, Johji
通讯作者：
Inazawa, Johji
影响因子：
6.4
作者：
Lee, Eun Jon;Gusev, Yuriy;Schmittgen, Thomas D.
通讯作者：
Schmittgen, Thomas D.
影响因子：
4.7
作者：
Langevin, Scott M.;Stone, Roslyn A.;Taioli, Emanuela
通讯作者：
Taioli, Emanuela