T-cell response profiling to biological threat agents including the SARS coronavirus
T-cell response profiling to biological threat agents including the SARS coronavirus
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DOI：
10.1177/039463200501800312
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发表时间：
2005-07-01
影响因子：
3.5
通讯作者：
Poccia, F
中科院分区：
文献类型：
--
作者：
Gioia, C;Horejsh, D;Poccia, F
病原体如SARS的出现和生物恐怖主义威胁的增加刺激了用于鉴别诊断的新型诊断测定的发展。我们开发了一种T细胞分析系统，以阵列形式监测对病原体的反应，而不是专注于检测单个病原体组分。使用对不同病原体具有特异性的抗原基质，通过流式细胞术监测细胞内干扰素-γ的产生来产生每个个体的特异性T细胞谱。该检测试剂盒可一次检测多种蛋白质或肽，并对CD 4（+）和CD 8（+）应答细胞进行定量和表型评估。我们目前分析的几个积极的个人，包括那些接种天花和炭疽疫苗的例子。我们还展示了SARS-hCoV的抗原优化，因为研究表明这些蛋白质含有与更常见的冠状病毒交叉反应的肽，这是普通感冒的原因。T细胞阵列是活动性感染、暴露于病原体或有效的近期疫苗接种的早期和敏感的多重测量。
The emergence of pathogens such as SARS and the increased threat of bioterrorism has stimulated the development of novel diagnostic assays for differential diagnosis. Rather than focusing on the detection of an individual pathogen component, we have developed a T cell profiling system to monitor responses to the pathogens in an array format. Using a matrix of antigens specific for different pathogens, a specific T cell profile was generated for each individual by monitoring the intracellular production of interferon-gamma by flow cytometry. This assay allows for the testing of multiple proteins or peptides at a single time and provides a quantitative and phenotypic assessment of CD4(+) and CD8(+) responding cells. We present profiling examples for several positive individuals, including those vaccinated with the smallpox and anthrax vaccines. We also show antigen optimization for the SARS-hCoV, as studies revealed that these proteins contain peptides which cross-react with more common coronaviruses, a cause,of the common cold. The T cell array is an early and sensitive multiplex measure of active infection, exposure to a pathogen, or effective, recent vaccination.