Expression analysis of PCSTE3, a putative pheromone receptor from the lung pathogenic fungus Pneumocystis carinii.
Expression analysis of PCSTE3, a putative pheromone receptor from the lung pathogenic fungus Pneumocystis carinii.
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PCSTE3 的表达分析，PCSTE3 是一种来自肺病原真菌卡氏肺囊虫的假定信息素受体。
DOI：
10.1016/j.bbrc.2004.04.154
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
ThomasJr,CharlesF
中科院分区：
文献类型：
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作者：
Vohra,PawanK;Park,JohnG;Sanyal,Bharati;ThomasJr,CharlesF
真菌病原体卡氏肺囊虫仍然是艾滋病毒感染者中最普遍的机会性感染。真菌信息素受体是7种跨膜结构域g蛋白偶联受体，它们在特定的交配类型上表达，并且对来自相反交配类型的细胞的特定信息素具有配体结合的胞外结构域。我们克隆并鉴定了carinii的PCSTE3，该蛋白编码与酿酒酵母信息素受体Ste3同源的7跨膜结构域蛋白。我们使用针对表达该蛋白的酵母受体胞外结构域设计的抗体，通过间接免疫荧光检测PCSTE3。使用下游的Fus1-lacZ报告基因，我们确定PCSTE3不识别a-或α-因子信息素作为受体的配体。我们分离了卡氏假单胞菌的生命周期阶段，并通过免疫荧光显微镜和流式细胞术检测了PCSTE3的表达，发现PCSTE3只在营养形式的群体中表达。囊肿未见PCSTE3受体表达。
The fungal pathogen Pneumocystis carinii remains the most prevalent opportunistic infection in patients infected with HIV. Fungal pheromone receptors are seven transmembrane domain G-protein-coupled receptors which are expressed on specific mating types, and have ligand-binding extracellular domains for specific pheromones from cells of the opposite mating type. We have cloned and characterized PCSTE3 from P. carinii, which encodes a seven transmembrane domain protein orthologous to the Saccharomyces cerevisiae pheromone receptor Ste3. We detect PCSTE3 by indirect immunofluorescence using antibodies designed to extracellular domains of the receptor in yeast expressing the protein. Using a downstream Fus1-lacZ reporter gene, we determined that PCSTE3 does not recognize a- or α-factor pheromones as ligands for the receptor. We isolated P. carinii life cycle stages and examined PCSTE3 expression by immunofluorescence microscopy and flow cytometry, and found PCSTE3 expression exclusively on a population of trophic forms. PCSTE3 receptor expression was not found on cysts.