The MAPKK kinase SteC regulates conidiophore morphology and is essential for heterokaryon formation and sexual development in the homothallic fungus Aspergillus nidulans
The MAPKK kinase SteC regulates conidiophore morphology and is essential for heterokaryon formation and sexual development in the homothallic fungus Aspergillus nidulans
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DOI：
10.1046/j.1365-2958.2003.03405.x
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发表时间：
2003-03
影响因子：
3.6
通讯作者：
Huijun Wei;N. Requena;R. Fischer
中科院分区：
文献类型：
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作者：
Huijun Wei;N. Requena;R. Fischer
环境信号可以通过MAP激酶级联反应的作用转化为细胞内反应。顺序磷酸化导致MAP激酶的瞬时激活，这反过来又激活某些转录因子，从而激活一组途径特异性基因。该级联反应中的许多步骤都是保守的，并且已经发现了从酵母到人类的同源物。我们的特点是MAPKK激酶，SteC，同源的酿酒酵母Ste 11，在丝状真菌构巢曲霉。长886个氨基酸的蛋白质与粗糙脉孢菌Nrc-1具有最高的相似性。A. nidulans导致生长速率减慢、形成更多分支的菌丝、改变分生孢子形态、抑制异核体形成和阻断闭囊壳的发育。该基因在无性发育过程中被转录激活，并控制两种假定的MAP激酶的磷酸化。
Environmental signals can be transduced into intracellular responses by the action of MAP kinase cascades. Sequential phosphorylation results in the transient activation of a MAP kinase, which in turn activates certain transcription factors and thus a set of pathway‐specific genes. Many steps in this cascade are conserved, and homologues have been discovered from yeast to human. We have characterized the MAPKK kinase, SteC, a homologue of Saccharomyces cerevisiae Ste11, in the filamentous fungus Aspergillus nidulans. The 886‐amino‐acid‐long protein shares the highest similarity to Neurospora crassa Nrc‐1. Deletion of the gene in A. nidulans results in a slower growth rate, the formation of more branched hyphae, altered conidiophore morphology, an inhibition of heterokaryon formation and a block of cleistothecium development. The gene is transcriptionally activated during asexual development and controls the phosphorylation of two putative MAP kinases.