Structure of an octameric form of the minichromosome maintenance protein from the archaeon Pyrococcus abyssi.
Structure of an octameric form of the minichromosome maintenance protein from the archaeon Pyrococcus abyssi.
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DOI：
10.1038/srep42019
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发表时间：
2017-02-08
影响因子：
4.6
通讯作者：
Spagnolo L
中科院分区：
文献类型：
--
作者：
Cannone G;Visentin S;Palud A;Henneke G;Spagnolo L
细胞分裂是一个复杂的过程，需要精确复制遗传物质。复制是由复制体协调的。微小染色体维持（MCM）复制解旋酶是复制体的重要组成部分。真核和古细菌MCM蛋白是高度保守的。事实上，古MCM是阐明MCM功能本质特征的有力工具。然而，虽然真核MCM 2 -7是由不同多肽链组成的异源复合物，但许多大肠杆菌的MCM复合物从单个基因产物形成同六聚体。此外，一些古细菌MCMs是多态性的，并且已经报道了相同多肽的六聚体和七聚体结构。在这里，我们展示了来自深海火球菌的古细菌MCM解旋酶的单八聚体环组装结构。据我们所知，这是第一个全长八聚体MCM解旋酶的报告。
Cell division is a complex process that requires precise duplication of genetic material. Duplication is concerted by replisomes. The Minichromosome Maintenance (MCM) replicative helicase is a crucial component of replisomes. Eukaryotic and archaeal MCM proteins are highly conserved. In fact, archaeal MCMs are powerful tools for elucidating essential features of MCM function. However, while eukaryotic MCM2-7 is a heterocomplex made of different polypeptide chains, the MCM complexes of many Archaea form homohexamers from a single gene product. Moreover, some archaeal MCMs are polymorphic, and both hexameric and heptameric architectures have been reported for the same polypeptide. Here, we present the structure of the archaeal MCM helicase from Pyrococcus abyssi in its single octameric ring assembly. To our knowledge, this is the first report of a full-length octameric MCM helicase.