Expansion of a unique macrophage subset in rheumatoid arthritis synovial lining layer
Expansion of a unique macrophage subset in rheumatoid arthritis synovial lining layer
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DOI：
10.1111/j.1365-2249.2008.03733.x
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发表时间：
2008-10-01
影响因子：
4.6
通讯作者：
Matsuyama, T.
中科院分区：
文献类型：
--
作者：
Tanaka, M.;Nagai, T.;Matsuyama, T.
Z39 Ig蛋白（C3 b和iC 3b的补体受体）在各种组织中的驻留组织巨噬细胞上表达。本研究旨在观察Z39 Ig+细胞在类风湿关节炎（RA）滑膜中的分布及其表型特征，并与骨关节炎（OA）和银屑病关节炎（PsA）滑膜进行比较。用Z39 Ig转染的小鼠前B细胞免疫小鼠，制备抗Z39 Ig单克隆抗体，用于鉴定Z39 Ig+细胞。采用免疫双染法对正常滑膜、RA滑膜、OA滑膜和PsA滑膜中的Z39 Ig+细胞进一步进行巨噬细胞、成纤维细胞样滑膜细胞、补体受体和树突状细胞的抗体染色。RA滑膜单个核细胞使用抗Z39 Ig和抗CD 11 c抗体双重染色，并分选为Z39 Ig + CD 11 c+细胞和Z39 Ig + CD 11 c-细胞。然后通过电子显微镜分析这些细胞群。Z39 Ig蛋白在正常、RA、OA和PsA滑膜中仅表达于内膜巨噬细胞。RA滑膜衬里层中Z39 Ig + CD 11 c+细胞数及Z39 Ig + CD 11 c+细胞与Z39 Ig+细胞的比值均高于OA和PsA。Z39 Ig + CD 11 c+细胞的超微结构分析显示巨噬细胞的特征，具有许多次级溶酶体和线粒体肿胀。Z39 Ig+细胞似乎可用于鉴定正常滑膜中的驻留组织巨噬细胞和炎性关节炎滑膜衬里层中的相应巨噬细胞。Z39 Ig + CD 11 c+细胞的扩增是RA滑膜衬里层的特征。
The Z39Ig protein (complement receptor for C3b and iC3b) is expressed on resident tissue macrophages in various tissues. This study was undertaken to examine the distribution of Z39Ig+cells and their phenotypic features in rheumatoid arthritis (RA) synovium, in comparison with those of osteoarthritis (OA) and psoriatic arthritis (PsA) synovium. Monoclonal anti-Z39Ig antibody was produced by immunizing Z39Ig transfected murine pre B cells and used for the identification of Z39Ig+cells. Z39Ig+cells were further stained with antibodies to macrophages, fibroblast-like synoviocytes, complement receptors and dendritic cells by using the double immunostaining method in normal, RA, OA and PsA synovium. RA synovial mononuclear cells were double-stained using anti-Z39Ig and anti-CD11c antibodies and sorted into Z39Ig+CD11c+cells and Z39Ig+CD11c-cells. These cell populations were then analysed by electron microscopy. The expression of the Z39Ig protein was limited to intimal macrophages in normal, RA, OA and PsA synovium. The numbers of Z39Ig+CD11c+cells and the ratios of Z39Ig+CD11c+cells to Z39Ig+cells were increased in the synovial lining layer of RA as compared with those of OA and PsA. The ultrastructural analysis of Z39Ig+CD11c+cells showed the character of macrophages with many secondary lysosomes and swelling of mitochondria. Z39Ig+ cells appeared to be useful for identification of resident tissue macrophages in normal synovium and the corresponding macrophages in the synovial lining layer of inflammatory arthritis. Expansion of Z39Ig+CD11c+cells was characteristic of RA synovial lining layer.