Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization
Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization
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DOI：
10.1016/j.fertnstert.2007.07.1390
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发表时间：
2007-11-01
影响因子：
6.7
通讯作者：
Burns, David H.
中科院分区：
文献类型：
--
作者：
Seli, Emre;Sakkas, Denny;Burns, David H.
目的：确定胚胎培养基的代谢组学分析是否与单个胚胎的生殖潜力相关。设计：前瞻性研究。设置：一个学术和私人辅助生殖项目;一个大学研究中心。患者：接受辅助生殖治疗的女性。干预：来自30名患者的69份已用培养基样本，结局已知在胚胎移植后第3天单独收集胚胎移植后的胚胎移植（0或100%持续着床率），并使用拉曼和/或近红外光谱进行评估。使用波长选择遗传算法分别分析从每个仪器获得的光谱，以确定预测妊娠结果的区域。计算每个样品反映生殖潜力的活力指数。为了避免随机相关性，使用留一交叉验证。计算预测存活率（描述为植入和分娩）的灵敏度和特异性。主要结果测量：培养基和胚胎存活率的代谢组学特征。结果：与植入失败的胚胎相比，通过拉曼或近红外光谱计算的存活率指数对于植入并导致分娩的胚胎更高。拉曼光谱预测单个胚胎存活率的灵敏度为86%，特异性为76.5%;近红外光谱预测单个胚胎存活率的灵敏度为75%，特异性为83.3%。结论：利用拉曼光谱或近红外光谱结合生物信息学技术快速、无创地分析人类胚胎培养液的代谢组学特征与妊娠结局相关。
Objective: To determine if metabolomic profiling of embryo culture media correlates with reproductive potential of individual embryos.Design: Prospective study.Setting: An academic and a private assisted reproduction program; a university research center.Patient(s): Women undergoing assisted reproduction treatment.Intervention(s): Sixty-nine spent media samples from 30 patients with known outcome (0 or 100% sustained implantation rates) were individually collected after embryo transfer on day 3 and were evaluated using Raman and/or near-infrared spectroscopy. The spectra obtained from each instrument were separately analyzed using a wavelength selective genetic algorithm to determine regions predictive of pregnancy outcome. Viability indices reflective of reproductive potential were calculated for each sample. To avoid random correlations, a leave-one-out cross-validation was used. Sensitivity and specificity of predicting viability (described as implantation and delivery) were calculated.Main Outcome Measure(s): Metabolomic profile of culture media and embryo viability.Result(s): Viability indices calculated by Raman or near-infrared spectroscopy were higher for embryos that implanted and resulted in a delivery, compared with those that failed to implant. Raman spectroscopy predicted viability of individual embryos with a sensitivity of 86% and a specificity of 76.5%; near-infrared provided a sensitivity of 75% and a specificity of 83.3%.Conclusion(s): Rapid, noninvasive metabolomic profiling of human embryo culture media using Raman or near-infrared spectroscopy combined with bioinformatics correlates with pregnancy outcome.