Microarray-Assisted Fine-Mapping of Quantitative Trait Loci for Cold Tolerance in Rice
Microarray-Assisted Fine-Mapping of Quantitative Trait Loci for Cold Tolerance in Rice
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DOI：
10.1093/mp/sss161
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发表时间：
2013-05-01
期刊：
影响因子：
27.5
通讯作者：
Sun, Chuanqing
中科院分区：
文献类型：
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作者：
Liu, Fengxia;Xu, Wenying;Sun, Chuanqing
许多重要的农艺性状，包括抗冷胁迫性，都很复杂，并受数量性状基因座 (QTL) 控制。分离这些QTL将极大地造福农业，但也是一项具有挑战性的任务。本研究探索了一种将微阵列与QTL作图相结合的综合策略，以鉴定耐冷品种IL112苗早期的耐冷QTL。 IL112的早期幼苗在4-5℃下均正常存活9天，而籼稻品种桂超2号(GC2)在相同条件下4天后死亡。使用源自 GC2 和 IL112 后代的 F-2:3 群体，我们鉴定了 7 个耐冷性 QTL。此外，我们进行了Affymetrix水稻全基因组芯片杂交，获得了IL112和GC2在低温和正常条件下的表达谱。基于微阵列数据挖掘和QTL作图，选择四个基因作为冷QTL相关候选基因。其中一个候选基因LOC_Os07g22494在多个水稻品种和F-2:3群体中被证明与耐寒性高度相关，其过表达转基因水稻植株在苗期表现出较强的低温耐受性。结果表明，该基因（LOC_Os07g22494）的过表达可以提高水稻幼苗的耐冷性。因此，本研究为鉴定确定的 QTL 区域中的候选基因提供了一种有前途的策略。
Many important agronomic traits, including cold stress resistance, are complex and controlled by quantitative trait loci (QTLs). Isolation of these QTLs will greatly benefit the agricultural industry but it is a challenging task. This study explored an integrated strategy by combining microarray with QTL-mapping in order to identify cold-tolerant QTLs from a cold-tolerant variety IL112 at early-seedling stage. All the early seedlings of IL112 survived normally for 9 d at 4-5 degrees C, while Guichao2 (GC2), an indica cultivar, died after 4 d under the same conditions. Using the F-2:3 population derived from the progeny of GC2 and IL112, we identified seven QTLs for cold tolerance. Furthermore, we performed Affymetrix rice whole-genome array hybridization and obtained the expression profiles of IL112 and GC2 under both low-temperature and normal conditions. Four genes were selected as cold QTL-related candidates, based on microarray data mining and QTL-mapping. One candidate gene, LOC_Os07g22494, was shown to be highly associated with cold tolerance in a number of rice varieties and in the F-2:3 population, and its overexpression transgenic rice plants displayed strong tolerance to low temperature at early-seedling stage. The results indicated that overexpression of this gene (LOC_Os07g22494) could increase cold tolerance in rice seedlings. Therefore, this study provides a promising strategy for identifying candidate genes in defined QTL regions.