Identification of an expanded set of translationally active methionine analogues in Escherichia coli
Identification of an expanded set of translationally active methionine analogues in Escherichia coli
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DOI：
10.1016/s0014-5793(01)02657-6
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发表时间：
2001-07-27
期刊：
影响因子：
3.5
通讯作者：
Tirrell, DA
中科院分区：
文献类型：
--
作者：
Kiick, KL;Weberskirch, R;Tirrell, DA
在体内，氨基酸掺入蛋白质中最严格地由氨酰-tRNA合成酶控制。在这里，我们报告了几个新的蛋氨酸类似物纳入蛋白质增加其激活率的甲硫氨酰-tRNA合成酶（MetRS）的大肠杆菌。顺式-巴豆基甘氨酸（4）、2-氨基庚酸（7）、正缬氨酸（8）、2-丁炔基甘氨酸（11）和烯丙基甘氨酸（12）将各自支持甲硫氨酸耗尽的大肠杆菌培养物中的蛋白质合成。当MetRS过表达并且培养基补充有毫摩尔浓度的类似物时，这些研究表明，蛋白质工程的重要机会，作为扩展的翻译装置向其他氨基酸类似物通过类似的策略也应该是可能的。(C)2001年由Elsevier Science B. V.代表欧洲生物化学学会联合会出版。
Amino acid incorporation into proteins in vivo is controlled most stringently by the aminoacyl-tRNA synthetases. Here we report the incorporation of several new methionine analogues into protein by increasing the rate of their activation by the methionyl-tRNA synthetase (MetRS) of Escherichia coli. cis-Crotylglycine (4), 2-aminoheptanoic acid (7), norvaline (8), 2-butynylglycine (11), and allylglycine (12) will each support protein synthesis in methionine-depleted cultures of E. coli when MetRS is overexpressed and the medium is supplemented with the analogue at millimolar concentrations. These investigations suggest important opportunities for protein engineering, as expansion of the translational apparatus toward other amino acid analogues by similar strategies should also be possible. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.