Effects of pH on ultrasonic-modified soybean lipophilic protein nanoemulsions with encapsulated vitamin E

Effects of pH on ultrasonic-modified soybean lipophilic protein nanoemulsions with encapsulated vitamin E
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pH值对超声波改性维生素E大豆亲脂蛋白纳米乳的影响

DOI:
10.1016/j.lwt.2021.111240
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发表时间:
2021-03
期刊:
LWT- Food Science and Technology
影响因子:
--
通讯作者:
Li Yang
Li Yang
中科院分区:
其他
文献类型:
--
作者:
Wang Diqiong;Zhong Mingming;Sun Yufan;Fang Lin;Sun Yu;a;Qi Baokun;Li Yang

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大豆亲脂蛋白(LP)是一种很有前途的纳米乳给药乳化剂。本文测定了pH对超声改性LP纳米乳液的稳定性、流变性和包封率的影响,并对其影响机理进行了探讨。该缓释体系在中性和碱性环境(pH 7.0或9.0)中具有较高的Zeta电位值(18-30 mV)和均匀的粒径分布(100-350 mV)。低温扫描电子显微镜显示,中性和碱性环境中的LP比酸性环境中的LP更均匀地覆盖油水界面。此外,该低密度脂蛋白纳米乳液具有良好的流变性,具有较高的乳化性能、维生素E包封率和氧化稳定性。这些改善是因为使用超声LP制备的纳米乳液具有高的绝对Zeta电位值(最大30 mV)、小的液滴尺寸(最小190 nm)、均匀的蛋白质膜、高粘度以及在不同pH环境下的良好粘弹性。综上所述,LP超声法制备的纳米乳剂给药系统可以抵抗不同pH环境的影响,在中性和碱性环境中最大限度地提高给药效率。研究结果确定了LP纳米乳液适宜的pH环境,为其进一步应用提供了理论参考。
Soybean lipophilic protein (LP) is a promising emulsifier for nanoemulsion delivery systems. Herein, the influence of pH on the stability, rheological properties, and encapsulation efficiency of a nanoemulsion formed by ultrasonically modified LP was determined, and its influence mechanism was explored. The delivery system exhibited a high absolute zeta potential value (18–30 mV) in neutral and alkaline environments (pH 7.0 or 9.0) and uniform particle size distribution (100–350 nm). Cryo-scanning electron microscopy showed that LPs in neutral and alkaline environments covered the oil–water interface more evenly than those in acidic environments. Moreover, the LP nanoemulsion has excellent rheological properties and exhibit high emulsification performance, vitamin E encapsulation efficiency, and oxidation stability. These improvements occurred because the nanoemulsion prepared using ultrasonicated LP had a high absolute zeta potential value (maximum 30 mV), small droplet size (minimum 190 nm), uniform protein film, high viscosity, and good viscoelasticity in different pH environments. In summary, the nanoemulsion delivery system prepared by LP ultrasonication can resist the influence of different pH environments and maximize the delivery efficiency in neutral and alkaline environments. The research results identified a suitable pH environment for LP nanoemulsions and provide a theoretical reference for their further application.
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