Structural alteration of a BYDV-like translation element (BTE) that attenuates p35 expression in three mild Tobacco bushy top virus isolates.
Structural alteration of a BYDV-like translation element (BTE) that attenuates p35 expression in three mild Tobacco bushy top virus isolates.
复制标题
BYDV 样翻译元件 (BTE) 的结构改变可减弱三种轻度烟草丛顶病毒分离株中 p35 的表达
DOI：
10.1038/s41598-017-04598-5
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发表时间：
2017-06-23
影响因子：
4.6
通讯作者：
Yuan X
中科院分区：
文献类型：
--
作者：
Wang D;Yu C;Liu S;Wang G;Shi K;Li X;Yuan X
为了确定烟草丛顶病毒（TBTV）进化对烟草丛顶病退化的分子效应，将3株轻度毒力TBTV分离株与野生型TBTV进行比较，以评估p35的翻译，p35依赖于一个不依赖于帽盖的bydv样翻译元件（BTE）。在轻度分离株中p35的体外表达仅为wt TBTV中所观察到的20%至40%。根据嵌合TBTV RNA的翻译数据，三个轻度分离株的p35低水平表达与两个区域有关：5 ‘末端500 nt区（RI）和3 ’内部区（RV），其中包括BTE。对于RV区，基于SHAPE结构探测和突变分析，p35的低水平表达主要与BTE的结构改变有关，而不是与BTE内特定序列突变有关。此外，TBTV BTE的结构改变是由BTE外部的突变引起的，这意味着BTE周围局部区域的结构复杂性。本研究首次报道了一种特定RNA病毒的不同分离株中与3 '帽无关的翻译元件的结构改变，这与病毒毒力的变化有关。
To identify the molecular effects of Tobacco bushy top virus (TBTV) evolution on the degeneration of tobacco bushy top disease, three TBTV isolates with mild virulence were compared with wild-type TBTV to assess the translation of p35, which relies on a BYDV-like translation element (BTE) in a cap-independent manner. The in vitro expression of p35 in the mild isolates was only 20% to 40% of the expression observed in wt TBTV. Based on translation data from chimeric TBTV RNA, low-level p35 expression in the three mild isolates was associated with two regions: the 5′ terminal 500 nt region (RI) and the 3′ internal region (RV), which included the BTE. For the RV region, low level p35 expression was mainly associated with structural alterations of the BTE instead of specific sequence mutations within the BTE based on SHAPE structural probing and mutation analysis. Additionally, structural alteration of the TBTV BTE resulted from mutations outside of the BTE, implying structural complexity of the local region surrounding the BTE. This study is the first report on the structural alteration of the 3′ cap-independent translation element among different isolates of a given RNA virus, which is associated with variations in viral virulence.