Primary characterization of bacteriocin paracin C - A novel bacteriocin produced by Lactobacillus paracasei
Primary characterization of bacteriocin paracin C - A novel bacteriocin produced by Lactobacillus paracasei
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细菌素 paracin C 的初步表征——一种由副干酪乳杆菌产生的新型细菌素
DOI：
10.1016/j.foodcont.2013.03.040
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发表时间：
2013-11-01
期刊：
影响因子：
6
通讯作者：
Yue, Tianli
中科院分区：
文献类型：
--
作者：
Pei, Jinjin;Yuan, Yahong;Yue, Tianli
副乳杆菌CICC 20241产生一种5 kDa的细菌素，命名为帕拉菌素C，对本研究中使用的17株脂环菌有活性。以A.acidoterrestris DSM 3922为目标菌株，在37℃的MRS发酵液中高水平生产帕拉星C，最高产量达1800AU/ml。帕拉星C的主要作用方式很可能是由于孔的形成，如代谢活性细胞K+的外流所表明的那样。在10 mM的Gd存在下，对羟基苯甲酸C不影响嗜酸拟青霉细胞。这表明，帕拉星C的作用方式依赖于净带负电荷的细胞表面。此外，还研究了不同的培养基组分对帕拉星C产量的影响。(C)爱思唯尔有限公司出版的2013年。
Lactobacillus paracasei CICC 20241 produces a 5 kDa bacteriocin, named paracin C, active against seventeen strains of Alicyclobacillus acidoterrestris used in this study. Paracin C was produced at high levels in MRS broth at 37 degrees C, reaching a maximum production of 1800 AU/ml using A. acidoterrestris DSM 3922 as target strain. The primary mode of action of paracin C is most probably due to pore formation, as indicated by the efflux of K+ from metabolically active cells of A. acidoterrestris. In the presence of 10 mM gadolinium, paracin C did not affect cells of A. acidoterrestris. This suggests that the mode of action of paracin C relies on a net negatively charged cell surface. In addition, effect of different medium components on paracin C production was studied. (C) 2013 Published by Elsevier Ltd.