Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.
Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche.
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DOI：
10.1038/nature07434
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发表时间：
2009-01-01
期刊：
影响因子：
64.8
通讯作者：
Scadden, David T.
中科院分区：
文献类型：
--
作者：
Lo Celso, Cristina;Fleming, Heather E.;Wu, Juwell W.;Zhao, Cher X.;Miake-Lye, Sam;Fujisaki, Joji;Cote, Daniel;Rowe, David W.;Lin, Charles P.;Scadden, David T.
干细胞存在于一个专门的，被称为生态位的调节环境中，决定了它们如何产生，维持和修复组织。我们先前已经证明，移植的造血干细胞和祖细胞群体定位于趋化因子CXCL 12特别丰富的骨髓微血管的亚域。使用高分辨率共聚焦显微镜和双光子视频成像的活小鼠颅骨骨髓中的单个造血细胞随着时间的推移相结合，我们研究造血干细胞和祖细胞的血管，成骨细胞和骨内膜表面的关系，因为它们的家和移植在辐照和c-Kit受体缺陷的受体小鼠。成骨细胞在微血管和干/祖细胞在这个复杂的组织中的相对定位是非随机和动态的。细胞自主和非自主因素影响原始细胞定位。不同的造血细胞亚群根据分化阶段定位于不同的位置。当生理挑战驱使植入或扩增时，骨髓干/祖细胞假定位置靠近骨和成骨细胞。我们的分析允许观察在真实的时间，在一个单一的细胞水平，以前已经研究过的过程，只有他们的长期结果在有机体水平。
Stem cells reside in a specialized, regulatory environment termed the niche that dictates how they generate, maintain and repair tissues. We have previously documented that transplanted haematopoietic stem and progenitor cell populations localize to subdomains of bone-marrow microvessels where the chemokine CXCL12 is particularly abundant. Using a combination of high-resolution confocal microscopy and two-photon video imaging of individual haematopoietic cells in the calvarium bone marrow of living mice over time, we examine the relationship of haematopoietic stem and progenitor cells to blood vessels, osteoblasts and endosteal surface as they home and engraft in irradiated and c-Kit-receptor-deficient recipient mice. Osteoblasts were enmeshed in microvessels and relative positioning of stem/progenitor cells within this complex tissue was nonrandom and dynamic. Both cell autonomous and non-autonomous factors influenced primitive cell localization. Different haematopoietic cell subsets localized to distinct locations according to the stage of differentiation. When physiological challenges drove either engraftment or expansion, bone-marrow stem/progenitor cells assumed positions in close proximity to bone and osteoblasts. Our analysis permits observing in real time, at a single cell level, processes that previously have been studied only by their long-term outcome at the organismal level.
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影响因子：
9.8
作者：
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通讯作者：
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影响因子：
64.5
作者：
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作者：
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作者：
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通讯作者：
Jacobsen, SEW
DOI：
10.1109/jstqe.2007.912751
发表时间：
2008-01-01
影响因子：
4.9
作者：
Veilleux, Israel;Spencer, Joel A.;Lin, Charles P.
通讯作者：
Lin, Charles P.