Analysis of a Blumeria graminis-Secreted Lipase Reveals the Importance of Host Epicuticular Wax Components for Fungal Adhesion and Development
Analysis of a Blumeria graminis-Secreted Lipase Reveals the Importance of Host Epicuticular Wax Components for Fungal Adhesion and Development
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DOI：
10.1094/mpmi-22-12-1601
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发表时间：
2009-12-01
影响因子：
3.5
通讯作者：
Wei, Yangdou
中科院分区：
文献类型：
--
作者：
Feng, Jie;Wang, Feng;Wei, Yangdou
活体营养型白粉病真菌Blumeria graminis将细胞外物质释放到真菌感染结构的表面，以便于在感染前将它们锚定到疏水植物表面;然而，真菌粘合剂的化学性质和粘合机制在很大程度上仍不清楚。表达序列标签分析导致从B鉴定出分泌的脂肪酶Lip 1。禾本科植物LIP 1的表达在真菌发育的早期阶段显著上调。Lip 1，分泌到真菌细胞壁的表面，具有对广泛的甘油酯的脂解活性，并从小麦叶的表皮蜡释放烷烃和伯脂肪醇。由Lip 1活性释放的表皮蜡组分中，长链烷烃是触发附着胞形成的最有效的线索。用Lip 1预处理小麦叶片，从而去除叶表面蜡，严重损害真菌致病性的组分，包括分生孢子粘附、附着胞形成和次生菌丝生长。我们的数据表明，Lip 1活性从宿主表面释放线索，以促进病原体的发展和感染。
The biotrophic powdery mildew fungus Blumeria graminis releases extracellular materials to the surface of fungal infection structures that facilitate anchoring them to hydrophobic plant surfaces prior to infection; however, the chemistry of fungal adhesives and the mechanism of adhesion remain largely unclear. Expressed sequence tag analysis led to identification of a secreted lipase, Lip1, from B. graminis. Expression of LIP1 is dramatically upregulated during the early stages of fungal development. Lip1, secreted to the surface of fungal cell walls, possesses lipolytic activity against a broad range of glycerides and releases alkanes and primary fatty alcohols from the epicuticular wax of wheat leaves. Of the epicuticular wax components released by Lip1 activity, long-chain alkanes are the most efficient cues for triggering appressorium formation. Pretreatment of wheat leaves with Lip1, thereby removing leaf surface wax, severely compromises components of fungal pathogenicity, including conidial adhesion, appressorium formation, and secondary hypha growth. Our data suggest that Lip1 activity releases cues from the host surface to promote pathogen development and infection.