Genetic variation of in vitro cytolytic activity and in vivo rejection potential of non‐immunized semi‐syngeneic mice against a mouse lymphoma line
Genetic variation of in vitro cytolytic activity and in vivo rejection potential of non‐immunized semi‐syngeneic mice against a mouse lymphoma line
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未免疫半同基因小鼠针对小鼠淋巴瘤系的体外细胞溶解活性和体内排斥潜力的遗传变异
DOI：
10.1002/ijc.2910150608
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发表时间：
1975
影响因子：
6.4
通讯作者：
H. Wigzell
中科院分区：
文献类型：
--
作者：
R. Kiessling;G. Petrányi;G. Klein;H. Wigzell
某些基因型的正常幼龄小鼠脾脏含有淋巴细胞，在51 Cr释放细胞毒性试验中，这些淋巴细胞可以杀死菌株A衍生的YAC-1和其他一些体外生长的Moloney淋巴瘤细胞系。我们之前已经证明，在本试验中，小鼠品系可以分为高反应者和低反应者。低反应性菌株和高反应性菌株之间的F1杂种具有高反应性。在本研究中，平行测试了A系小鼠和8种不同的A F1杂交小鼠的脾细胞介导的体外杀伤作用及其体内排斥分级数量的YAC腹水或体外培养细胞的能力。在所有测试的基因型中，体外细胞毒性和体内排斥反应之间存在明显的相关性。在分离（A × C57 Bl）× A回交小鼠中，YAC细胞的体内排斥反应与H-2相关。这与早期的体外细胞毒性回交分析一致，表明多基因对照至少有一个H-2连锁因子。
Spleens of normal young mice of certain genotypes contain lymphocytes that can kill strain A‐derived YAC‐1 and some other in vitro‐grown Moloney lymphoma lines in a 51Cr‐release cytotoxic test. We have previously shown that mouse strains can be classified as high or low reactors in this test. F1 hybrids between low‐ and high‐reactive strains are high‐reactive. In the present study, strain‐A mice and eight different A F1 hybrids were tested in parallel for their spleen‐cell‐mediated killing effect in vitro and their ability to reject graded numbers of YAC ascites or in vitro cultivated cells in vivo. There was a clear correlation between in vitro cytotoxicity and in vivo rejection in all tested genotypes. In segregating (A × C57Bl) × A backcross mice, in vivo rejection of YAC cells was H‐2 linked. This is in line with the earlier backcross analysis of the in vitro cytotoxicity, suggesting a polygenic control with at least one H‐2 linked factor.