Metabolic Syndrome is Associated with Ceramide Accumulation in Visceral Adipose Tissue of Women with Morbid Obesity
Metabolic Syndrome is Associated with Ceramide Accumulation in Visceral Adipose Tissue of Women with Morbid Obesity
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DOI：
10.1002/oby.22405
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发表时间：
2019-03-01
期刊：
影响因子：
6.9
通讯作者：
Miklosz, Agnieszka
中科院分区：
文献类型：
--
作者：
Choromanska, Barbara;Mysliwiec, Piotr;Miklosz, Agnieszka
目的长链脂肪酸依赖于脂肪酸转运蛋白的加速跨膜转运是导致脂质蓄积并最终导致代谢综合征的原因。本研究测定了脂质的含量（神经酰胺[CER]，二酰基甘油[DAG]，三酰基甘油，和游离脂肪酸[FFA]）和脂肪酸转位酶的表达无代谢综合征（MetSx-）或代谢综合征（MetSx+）的病态肥胖女性内脏脂肪组织（VAT）和皮下脂肪组织中的FAT/CD 36和质膜脂肪酸结合蛋白并将结果与没有代谢综合征的瘦对照组进行比较。方法采用气相色谱法测定各脂质亚类的脂质含量和脂肪酸组成。对于总的，质膜，和线粒体的脂肪酸转运蛋白的表达，亚分级与随后的蛋白质印迹技术。结果肥胖组（MetSx-）VAT中甘油三酯含量高于对照组（P < 0. 05），而MetSx+组和精瘦组VAT中CER含量高于对照组（P < 0. 05）。这伴随着MetSx+受试者中VAT中FAT/CD 36的总表达和膜表达增加。因此，在两组肥胖受试者中，FAT/CD 36和质膜脂肪酸结合蛋白的线粒体表达均降低。结论代谢综合征与VAT中CER的积累有关，可能与FAT/CD 36蛋白表达增加有关。
Objective Accelerated transmembrane transport of long-chain fatty acids dependent on fatty acid transporters is responsible for lipid accumulation and, eventually, the development of metabolic syndrome. This study determined the content of lipids (ceramide [CER], diacylglycerol [DAG], triacylglycerol, and free fatty acid [FFA]) and the expression of fatty acid translocase (FAT/CD36) and plasma membrane fatty acid-binding protein in visceral adipose tissue (VAT) and subcutaneous adipose tissue of women with morbid obesity without metabolic syndrome (MetSx-) or with metabolic syndrome (MetSx+) and compared the results with those of lean controls without metabolic syndrome. Methods Lipid content and fatty acid composition in each lipid subclass were estimated by gas liquid chromatography. For total, plasma membrane, and mitochondrial expression of fatty acid transporters, subfractionation with subsequent Western blot technique was used. Results A greater content of triacylglycerol in VAT of participants with obesity (MetSx-) was found. However, only the MetSx+ subjects had increased content of CER in VAT in relation to subcutaneous adipose tissue in MetSx+ and lean individuals. This was accompanied by increased total and membrane expression of FAT/CD36 in VAT in MetSx+ subjects. Accordingly, mitochondrial expression of FAT/CD36 and plasma membrane fatty acid-binding protein was decreased in both groups of subjects with obesity. Conclusions Metabolic syndrome is associated with the accumulation of CER in VAT, possibly related to increased FAT/CD36 protein expression.