Effect of free-living daily physical activity on salivary secretory IgA in elderly
Effect of free-living daily physical activity on salivary secretory IgA in elderly
复制标题
DOI：
10.1249/mss.0b013e318031306d
复制
发表时间：
2007-04-01
期刊：
影响因子：
--
通讯作者：
Kono, Ichiro
中科院分区：
文献类型：
--
作者：
Shimizu, Kazuhiro;Kimura, Fuminori;Kono, Ichiro
目的：这项工作的目的是评估老年受试者自由生活的日常体力活动与粘膜免疫之间的关系，特别是唾液分泌性免疫球蛋白 A (SIgA)。方法：年龄 71.3 +/- 0.3 岁（范围：65-86 岁）的老年志愿者（114 名男性和 170 名女性）参与了本研究。早上采集静息唾液样本。以 60/60 秒的频率咀嚼无菌棉球刺激唾液样本。采用酶联免疫吸附测定(ELISA)测定SIgA浓度，并计算SIgA分泌率。使用电动计步器评估自由生活步数（每天步数）、能量消耗（kJ(.)kg(-1)-d(-1)）和特定强度水平（不活动、轻度、中度和剧烈）的活动持续时间（min(.)d(-1)）。获得的数据按照计步器确定的每天步数进行分层，并使用四分位数（Q1-Q4）进行分布。结果：四分位老年人每天的步数为 2962 94、5118 62、6832 59 和 9951 264 步。随着计步器确定的活动四分位数的增加，平均步数 (P < 0.0001)、能量消耗 (P < 0.0001) 和活动持续时间 (P < 0.0001) 存在显着差异。 Q3 的 SIgA 浓度和 SIgA 分泌率均显着高于 Q1 (P < 0.05)。与此同时，四分位数之间的唾液流量没有显着差异。结论：这些结果表明，每天约7000步的自由生活日常体力活动水平可能被视为老年人改善粘膜免疫功能的适度日常体力活动目标。
Purpose: The aim of this work was to assess the relationship in elderly subjects between free-living daily physical activity and mucosal immunity, especially salivary secretory immunoglobulin A (SIgA). Methods: Elderly volunteers (114 men and 170 women) aged 71.3 +/- 0.3 yr (range: 65-86 yr) participated in this study. Resting saliva samples were collected in the morning. Saliva samples stimulated by chewing a sterile cotton ball at a frequency of 60/60 s were collected. The SIgA concentration was measured using enzyme-linked immunosorbent assay (ELISA), and the SIgA secretion rate was calculated. Free-living step count (steps per day), energy expenditure (kJ(.)kg(-1)-d(-1)), and activity durations (min(.)d(-1)) at specific intensity levels (inactive, light, moderate, and vigorous) were evaluated using an electric pedometer. The data obtained were stratified by pedometer-determined steps per day using quartiles (Q1-Q4) for distribution. Results: Elderly in quartiles showed step counts of 2962 94, 5118 62, 6832 59, and 9951 264 steps per day. Significant differences were found in the mean step count (P < 0.0001), energy expenditure (P < 0.0001), and activity duration (P < 0.0001) with increasing pedometer-determined activity quartiles. Both SIgA concentration and SIgA-secretion rate were significantly higher for Q3 than for Q1 (P < 0.05). Meanwhile, saliva flow rates showed no significant differences across quartiles. Conclusion: These results suggest that a free-living daily physical activity level of approximately 7000 steps per day might be regarded as a moderate daily physical activity target for elderly people to improve mucosal immune function.