Teflon-carbon black as new material for the hydrophobic patterning of polymer labs-on-a-chip
Teflon-carbon black as new material for the hydrophobic patterning of polymer labs-on-a-chip
复制标题
聚四氟乙烯-炭黑作为聚合物芯片实验室疏水图案的新材料
DOI：
10.1109/sensor.2009.5285661
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
P. Koltay1
中科院分区：
文献类型：
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作者：
L. Riegger;M. Mielnik;D. Mark;W. Streule;M. Clad;R. Zengerle;P. Koltay1
我们提供了一种使用疏水性含氟聚合物对微流控芯片进行选择性表面图案化的新方法，该方法通过疏水性阀门的制造得到了证明。它可以对表面图案进行有效的光学质量控制，从而可以低成本生产高度可重复的疏水阀。具体来说，提出了一种基于炭黑（CB）的含氟聚合物-溶剂-染料溶液，它可以在由环烯烃共聚物（COC）制成的芯片上产生超疏水表面（接触角= 157.9°）。它还为聚合物芯片实验室中的质量控制 (QC) 提供了良好的可视性，并增加了疏水阀的爆破压力。最后，提出了一种旨在扩增芯片上 mRNA 并依赖于疏水阀定义的流量控制的应用。在此，与单独采用 Teflon-CB 涂层的阀门相比，QC 与 Teflon-CB 涂层相结合可将爆破压力的平均标准偏差从 14.5% 降低至 6.1%。
We provide a new method for the selective surface patterning of microfluidic chips with hydrophobic fluoropolymers which is demonstrated by the fabrication of hydrophobic valves. It enables efficient optical quality control for the surface patterning thus permitting the low-cost production of highly reproducible hydrophobic valves. Specifically, a fluoropolymer-solvent-dye solution based on carbon black (CB) is presented which creates superhydrophobic surfaces (contact angle = 157.9°) on chips made from cyclic olefin copolymer (COC). It further provides good visibility for the quality control (QC) in polymer labs-on-a-chip and increases the burst pressure of hydrophobic valves. Finally, an application which aims for the amplification of mRNA on-chip and relies on the defined flow control by hydrophobic valves is presented. Here, the QC in combination with the Teflon-CB coating improves the average standard deviation of the burst pressures from 14.5% down to 6.1 % compared to solely Teflon-coated valves.