A common rule governing differentiation kinetics of mouse cortical progenitors.
A common rule governing differentiation kinetics of mouse cortical progenitors.
复制标题
控制小鼠皮质祖细胞分化动力学的共同规则。
DOI：
10.1073/pnas.1916665117
复制
发表时间：
2020
影响因子：
11.1
通讯作者：
Stevens,CharlesF
中科院分区：
文献类型：
--
作者：
Sahara,Setsuko;Kodama,Takashi;Stevens,CharlesF
干细胞和祖细胞的增殖和分化之间的平衡决定了成年人大脑区域的大小。虽然调节皮质祖细胞增殖和分化的分子机制已被深入研究，但由于技术上的困难，对体内自我更新和分化之间的祖细胞选择动力学的分析仍然是未知的。在这里，我们建立了一个描述性的数学模型来估计自我更新的概率或分化的皮质祖细胞的行为在体内，一个变量，我们称之为膨胀系数。我们已经应用的模型，它只依赖于实验测量的参数，发展中的小鼠皮层，其中膨胀的神经上皮细胞和神经源性放射状胶质祖细胞共存。令人惊讶的是，我们发现，神经上皮细胞和放射状胶质祖细胞的膨胀系数遵循相同的发展轨迹，在皮质发育，这表明一个共同的规则管理自我更新/分化行为在小鼠皮质祖细胞分化。
The balance between proliferation and differentiation of stem cells and progenitors determines the size of an adult brain region. While the molecular mechanisms regulating proliferation and differentiation of cortical progenitors have been intensively studied, an analysis of the kinetics of progenitor choice between self-renewal and differentiation in vivo is, due to the technical difficulties, still unknown. Here we established a descriptive mathematical model to estimate the probability of self-renewal or differentiation of cortical progenitor behaviors in vivo, a variable we have termed the expansion coefficient. We have applied the model, one which depends only on experimentally measured parameters, to the developing mouse cortex where the expansive neuroepithelial cells and neurogenic radial glial progenitors are coexisting. Surprisingly, we found that the expansion coefficients of both neuroepithelium cells and radial glial progenitors follow the same developmental trajectory during cortical development, suggesting a common rule governing self-renewal/differentiation behaviors in mouse cortical progenitor differentiation.