Leucine-rich repeat C4 protein is involved in nervous tissue development and neurite outgrowth, and induction of glioma cell differentiation
Leucine-rich repeat C4 protein is involved in nervous tissue development and neurite outgrowth, and induction of glioma cell differentiation
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富含亮氨酸的重复 C4 蛋白参与神经组织发育和神经突生长，以及神经胶质瘤细胞分化的诱导。
DOI：
10.1111/j.1745-7270.2007.00338.x
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发表时间：
2007-10-01
影响因子：
3.7
通讯作者：
Li, Guiyuan
中科院分区：
文献类型：
--
作者：
Wu, Minghua;Huang, He;Li, Guiyuan
LRRC4是一种富含亮氨酸的重复C4蛋白，已在人类中被鉴定出来(GenBank登录号：AF196976)，小鼠(GenBank登录号：DQ177325)，大鼠(GenBank登录号：DQ119102)和牛(GenBank登录号：DQ164537)具有相同的域。就其相似性而言，这四种哺乳动物中编码LRRC4的基因是正交的，因此对应于同一基因实体。在前期研究的基础上，通过原位杂交，我们发现LRRC4在海马CA1和CA2、齿状回颗粒细胞、丘脑中背核和小脑浦肯野细胞层中表达最强。通过P19细胞模型，我们还发现LRRC4参与神经元和胶质细胞的分化。此外，含有LRR盒和免疫球蛋白结构域的细胞外蛋白已被证明参与轴突引导。我们的神经突生长实验数据表明，LRRC4促进海马神经元的神经突延伸，并诱导胶质母细胞瘤U251细胞向星形细胞样细胞分化，这一点通过形态学观察和胶质原纤维酸性蛋白表达得到证实。
LRRC4, leucine-rich repeat C4 protein, has been identified in human (GenBank accession No. AF196976), mouse (GenBank accession No. DQ177325), rat (GenBank accession No. DQ119102) and bovine (GenBank accession No. DQ164537) with identical domains. In terms of their similarity, the genes encoding LRRC4 in these four mammalian species are orthogs and therefore correspond to the same gene entity. Based on previous research, and using in situ hybridization, we found that LRRC4 had the strongest expression in hippocampal CA1 and CA2, the granule cells of the dentate gyrus region, the mediodoral thalamic nucleus, and cerebella Purkinje cell layers. Using a P19 cell model, we also found that LRRC4 participates in the differentiation of neuron and glia cells. In addition, extracellular proteins containing both an LRR cassette and immunoglobulin domains have been shown to participate in axon guidance. Our data from neurite outgrowth assays indicated that LRRC4 promoted neurite extension of hippocampal neurons, and induced differentiation of glioblastoma U251 cells into astrocyte-like cells, confirmed by morphology observation and glial fibrillary acidic protein expression.