Proteome profiling of Populus euphratica Oliv.: Upon heat stress
Proteome profiling of Populus euphratica Oliv.: Upon heat stress
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DOI：
10.1093/aob/mcl106
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发表时间：
2006-08-01
期刊：
影响因子：
4.2
通讯作者：
Pais, Maria Salome
中科院分区：
文献类型：
--
作者：
Ferreira, Silvia;Hjerno, Karin;Pais, Maria Salome
胡杨林是一种以先锋为特征的需光生态树种。它生长在沿着的防护林中，是中国和中东国家天然沙漠森林生态系统的一部分。它能够在极端温度、干旱和盐胁迫下存活，是研究木本植物在热胁迫下存活机制的重要植物物种。9方法通过测定叶片电解质渗漏量来评价热效应，确定LT 50发生在50 ℃以上。通过2D-PAGE确定了在人工气候室中42/37 ℃下处理3 d的幼苗叶片的蛋白质积累谱，共检测到45%的上调和下调蛋白质。基质辅助激光解吸电离飞行时间（MALDI-TOF）/TOF分析，结合在不同的数据库中的搜索，使识别82%的选定spots.Key结果短期上调蛋白质与膜不稳定和细胞骨架重构，硫同化，硫胺素和疏水性氨基酸的生物合成，和蛋白质的稳定性。长期上调的蛋白质参与氧化还原稳态和光合作用。结论中度热反应涉及与脂质合成、细胞骨架结构、硫酸盐同化、硫胺素和疏水性氨基酸生物合成以及核运输等相关的蛋白质。光稳定是通过碳代谢调节，减少光系统II（PSII）的丰度和增加PSI的光合线性电子流的贡献。硫氧还蛋白h可能在中度热暴露的胡杨的这一过程中发挥特殊作用。
Background and Aims Populus euphratica is a light-demanding species ecologically characterized as a pioneer. It grows in shelter belts along riversides, being part of the natural desert forest ecosystems in China and Middle Eastern countries. It is able to survive extreme temperatures, drought and salt stress, marking itself out as an important plant species to study the mechanisms responsible for survival of woody plants under heat stress. 9Methods Heat effects were evaluated through electrolyte leakage on leaf discs, and LT50 was determined to occur above 50 degrees C. Protein accumulation profiles of leaves from young plants submitted to 42/37 degrees C for 3 d in a phytotron were determined through 2D-PAGE, and a total of 45% of up- and downregulated proteins were detected. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)/TOF analysis, combined with searches in different databases, enabled the identification of 82% of the selected spots.Key Results Short-term upregulated proteins are related to membrane destabilization and cytoskeleton restructuring, sulfur assimilation, thiamine and hydrophobic amino acid biosynthesis, and protein stability. Long-term upregulated proteins are involved in redox homeostasis and photosynthesis. Late downregulated proteins are involved mainly in carbon metabolism.Conclusions Moderate heat response involves proteins related to lipid biogenesis, cytoskeleton structure, sulfate assimilation, thiamine and hydrophobic amino acid biosynthesis, and nuclear transport. Photostasis is achieved through carbon metabolism adjustment, a decrease of photosystem II (PSII) abundance and an increase of PSI contribution to photosynthetic linear electron flow. Thioredoxin h may have a special role in this process in P. euphratica upon moderate heat exposure.