GENERATION OF ALLOSPECIFIC NATURAL-KILLER-CELLS BY STIMULATION ACROSS A POLYMORPHISM OF HLA-C
GENERATION OF ALLOSPECIFIC NATURAL-KILLER-CELLS BY STIMULATION ACROSS A POLYMORPHISM OF HLA-C
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DOI：
10.1126/science.8493555
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发表时间：
1993-05-21
期刊：
影响因子：
56.9
通讯作者：
STROMINGER, JL
中科院分区：
文献类型：
--
作者：
COLONNA, M;BROOKS, EG;STROMINGER, JL
人自然杀伤（NK）细胞的细胞毒性由靶细胞表面上的主要组织相容性复合体人白细胞抗原（HLA）-C分子调节。对NK-1同种异体抗原特异性的同种异体反应性NK细胞可以通过用对HLA-C（Ser 77，Asn 80）纯合的靶细胞刺激而从对HLA-C（残基77处具有天冬酰胺并且在残基80处具有赖氨酸[HLA-C（Asn 77，Lys 80）]纯合的个体中可再现地产生;相互刺激产生对NK-2同种异体抗原特异性的NK细胞。然而，无论是纯合子靶细胞刺激异源反应性NK细胞的杂合子个体的产生。因此，这些数据揭示了由该系统定义的人NK同种异体反应性与鼠“杂交抗性”之间的意外差异。''
The cytotoxicity of human natural killer (NK) cells is modulated by the major histocompatibility complex human leukocyte antigen (HLA)-C molecules on the surface of the target cell. Alloreactive NK cells specific for the NK-1 alloantigen could be reproducibly generated from individuals that were homozygous for HLA-C with asparagine at residue 77 and lysine at residue 80 [HLA-C(Asn77, Lys80] by stimulation with target cells that were homozygous for HLA-C(Ser77, Asn80); the reciprocal stimulation yielded NK cells specific for the NK-2 alloantigen. However, neither homozygous target cell stimulated the generation of alloreactive NK cells from heterozygous individuals. Thus, these data reveal an unanticipated difference between human NK alloreactivity defined by this system and murine ''hybrid resistance.''