Expression of SoxC Transcription Factors during Zebrafish Retinal and Optic Nerve Regeneration
Expression of SoxC Transcription Factors during Zebrafish Retinal and Optic Nerve Regeneration
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斑马鱼视网膜和视神经再生过程中 SoxC 转录因子的表达
DOI：
10.1007/s12264-016-0073-2
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发表时间：
2017-02-01
影响因子：
5.6
通讯作者：
Xu, Hui
中科院分区：
文献类型：
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作者：
Mu, Zhaoxia;Zhang, Shuqiang;Xu, Hui
SoxC转录因子（Sox4、Sox11和Sox12）在脊椎动物眼睛和视网膜的发育中起重要作用。然而，它们在视网膜和视神经再生过程中的表达和功能仍然是难以捉摸的。在这项研究中，我们研究了成年斑马鱼视网膜和视神经损伤后SoxC基因的表达和可能的功能。我们发现，在5个SoxC成员中，Sox11b在视网膜损伤后的内核层（INL）中的BrdU阳性细胞中被强烈诱导，并且在损伤后4天，吗啉代介导的Sox11b敲低显著减少了INL中增殖细胞的数量。视神经损伤后，Sox11a和Sox11b在视网膜神经节细胞（retinal ganglion cells，RGC）中均呈强表达，敲低Sox11a和Sox11b可抑制RGC轴突的再生。因此，我们的研究揭示了视网膜和视神经损伤后SoxC家族基因的新表达模式，并表明它们在视网膜和视神经再生过程中具有重要功能。
The SoxC transcription factors (Sox4, Sox11, and Sox12) play important roles in the development of the vertebrate eye and retina. However, their expression and function during retinal and optic nerve regeneration remain elusive. In this study, we investigated the expression and possible functions of the SoxC genes after retinal and optic nerve injury in adult zebrafish. We found that among the five SoxC members, Sox11b was strongly induced in BrdU-positive cells in the inner nuclear layer (INL) after retinal injury, and morpholino-mediated Sox11b-knockdown significantly reduced the number of proliferating cells in the INL at 4 days post-injury. After optic nerve lesion, both Sox11a and Sox11b were strongly expressed in retinal ganglion cells (RGCs), and knockdown of both Sox11a and Sox11b inhibited RGC axon regrowth in retinal explants. Our study thus uncovered a novel expression pattern of SoxC family genes after retinal and optic nerve injury, and suggests that they have important functions during retinal and optic nerve regeneration.