Radiolabeled choline as a proliferation marker: comparison with radiolabeled acetate
Radiolabeled choline as a proliferation marker: comparison with radiolabeled acetate
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DOI：
10.1016/j.nucmedbio.2004.05.002
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发表时间：
2004-10-01
影响因子：
3.1
通讯作者：
Fujibayashi, Y
中科院分区：
文献类型：
--
作者：
Yoshimoto, M;Waki, A;Fujibayashi, Y
[C-11]胆碱是检测肿瘤的潜在示踪剂，特别是脑癌和前列腺癌。[C-11]胆碱的代谢定义了由正电子发射断层扫描描绘的肿瘤中[C-11]胆碱的积聚模式。胆碱是磷脂酰胆碱的前体，磷脂酰胆碱是膜脂质的主要成分。在细胞增殖过程中，膜脂质合成以及DNA合成被激活。我们使用10个肿瘤细胞系和成纤维细胞研究[C-14]胆碱代谢和增殖活性之间的关系。[C-14]胆碱摄取在肿瘤细胞中高于成纤维细胞，并且与增殖活性相关，尽管[C-14]胆碱摄取对增殖活性的敏感性低于[1-C-14]乙酸盐。[C-14]胆碱通过磷酸化产生的[C-14]磷酸胆碱主要促成了这种积累。[C-11]胆碱可通过估计胆碱激酶活性用于评价肿瘤增殖。(C)2004年爱思唯尔公司All rights reserved.
[C-11]Choline is a potential tracer to detect tumors, especially brain and prostate cancers. The metabolism of [C-11]choline defines the accumulation pattern of [C-11]choline in tumors depicted by positron emission tomography. Choline is a precursor of phosphatidylcholine that is a major constituent of membrane lipids. Membrane lipid synthesis as well as DNA synthesis is activated during cell proliferation. We investigated the relation between [C-14]choline metabolism and proliferative activity using 10 tumor cell lines and fibroblasts. [C-14] Choline uptake was higher in tumor cells than in fibroblasts and was correlated with the proliferative activity, though the sensitivity of [C-14] choline uptake to proliferative activity was less than that of [1-C-14]acetate. [C-14]Phosphocholine produced from [C-14]choline by phosphorylation mainly contributed to this accumulation. [C-11]Choline can be used for the evaluation of tumor proliferation through estimating choline kinase activity. (C) 2004 Elsevier Inc. All rights reserved.