Interferon-gamma potentiation of lipopolysaccharide-induced eicosanoid release from human monocytes.
Interferon-gamma potentiation of lipopolysaccharide-induced eicosanoid release from human monocytes.
复制标题
干扰素-γ增强脂多糖诱导的人单核细胞释放类二十烷酸。
DOI：
10.1089/jir.1987.7.121
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Garrison,SW
中科院分区：
文献类型：
--
作者：
Nichols,FC;Garrison,SW
干扰素-γ(干扰素-γ)可增强脂多糖刺激的单核巨噬细胞过程，包括释放白细胞介素1和杀瘤活性。本研究检测了干扰素-γ对内毒素刺激的人单核细胞释放前列腺素E_2(PGE_2)和血栓素B_2(T_XB_2)的调节能力。比较先用干扰素-γ孵育后再用脂多糖处理的细胞和同时用干扰素-γ和脂多糖处理的细胞释放PGE_2和TxB_2。在内毒素(10μg/ml，鼠伤寒沙门氏菌)刺激细胞之前加入干扰素-PGE2可使单核细胞释放前列腺素E(免疫法)和[~3H]PGE_2的量增加。相反，干扰素-γ预处理不能增强内毒素处理的单核细胞释放标记或免疫活性的TxB2。在不加内毒素刺激的情况下，干扰素-γ预处理不会导致二十烷类化合物的释放增加。此外，单核细胞同时加入干扰素-γ和脂多糖后，其释放的前列腺素E_2和T_xB_2的量并不比干扰素-γ和脂多糖单独作用的总和大。这些结果表明，干扰素-γ选择性地增强内毒素刺激的人单核细胞花生四烯酸转化为PGE_2，而不是T_xB_2。这种作用仅在单核细胞经脂多糖刺激前用干扰素-γ处理后才能观察到。
Interferon-γ (IFN-γ) can act to potentiate lipopolysaccharide (LPS)-stimulated processes in mononuclear phagocytes, including interleukin-1 release and tumoricidal activity. The present investigation examined the capacity of IFN-γ to modulate LPS-stimulated prostaglandin E2(PGE2) and thromboxane B2(TxB2) release from counterflow isolated human monocytes. The release of PGE2and TxB2was compared for cells incubated with IFN-γ prior to treatment with LPS and for cells treated simultaneously with IFN-γ and LPS. Treatment of cells with IFN-γ prior to stimulation with LPS (10 μg/ml,Salmonella typhimurium) resulted in elevated prostaglandin E (by immunoassay) and [3H]PGE2release from monocytes when compared with LPS-treated cultures. In contrast, IFN-γ pretreatment did not potentiate labeled or immunoreactive TxB2release from LPS-treated monocytes. IFN-γ pretreatment without LPS stimulation did not result in elevated eicosanoid release over controls. In addition, continuous treatment of monocytes with both IFN-γ and LPS did not result in greater release of PGE2and TxB2than the summed individual effects of IFN-γ and LPS. These results indicate that IFN-γ selectively potentiates LPS-stimulated arachidonic acid conversion to PGE2and not TxB2in human monocytes. This effect was observed only for monocytes pretreated with IFN-γ prior to stimulation with LPS.