Vertical distribution of airborne bacterial communities in an Asian dust downwind area, Noto Peninsula
Vertical distribution of airborne bacterial communities in an Asian dust downwind area, Noto Peninsula
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能登半岛亚洲沙尘顺风区空气细菌群落的垂直分布
DOI：
10.1016/j.atmosenv.2015.08.052
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发表时间：
2015
影响因子：
5
通讯作者：
Masah
中科院分区：
文献类型：
--
作者：
Bin Chen;Guang-Yu Shi;Maromu Yamada;Dai-Zhou Zhang;Masah
细菌种群从地面环境转移到大气中，分散在整个顺风区，可以影响生态系统动态、人类健康和气候变化。然而，对自由对流层中细菌垂直分布的详细研究却很少。我们在日本诺托半岛上空3000米、1000米和10米的高度收集气溶胶，那里的西风携带来自大陆和海洋地区的气溶胶。2012年3月10日采样期间，3000 m的气团被西风从中国沙漠地区输送过来，在2000 m以下形成边界层。针对16S rRNA基因（16S rDNA）的焦氧测序显示，3000 m的细菌群落主要由陆生细菌组成，如芽孢杆菌和放线菌。相比之下，在1000米和10米的海洋细菌属于蓝藻菌和阿尔法变形菌纲。克隆文库中的16S rDNA序列与原产于中国沙漠地区的陆生细菌和原产于日本海的海洋细菌完全相同。气团的来源和气象条件对下风大气中细菌群落的垂直变化有一定的影响。
Bacterial populations transported from ground environments to the atmosphere get dispersed throughout downwind areas and can influence ecosystem dynamics, human health, and climate change. However, the vertical bacterial distribution in the free troposphere was rarely investigated in detail. We collected aerosols at altitudes of 3000 m, 1000 m, and 10 m over the Noto Peninsula, Japan, where the westerly winds carry aerosols from continental and marine areas. During the sampling period on March 10, 2012, the air mass at 3000 m was transported from the Chinese desert region by the westerly winds, and a boundary layer was formed below 2000 m. Pyrosequencing targeting 16S rRNA genes (16S rDNA) revealed that the bacterial community at 3000 m was predominantly composed of terrestrial bacteria, such asBacillusandActinobacteriumspecies. In contrast, those at 1000 m and 10 m included marine bacteria belonging to the classesCyanobacteriaandAlphaproteobacteria. The entire 16S rDNA sequences in the clone libraries were identical to those of the terrestrial and marine bacterial species, which originated from the Chinese desert region and the Sea of Japan, respectively. The origins of air masses and meteorological conditions contribute to vertical variations in the bacterial communities in downwind atmosphere.