Expression of Wnt/β-catenin signaling, stem-cell markers and proliferating cell markers in rat whisker hair follicles
Expression of Wnt/β-catenin signaling, stem-cell markers and proliferating cell markers in rat whisker hair follicles
复制标题
大鼠胡须毛囊中 Wnt/β-catenin 信号、干细胞标志物和增殖细胞标志物的表达
DOI：
10.1007/s10735-015-9616-5
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发表时间：
2015-06-01
影响因子：
3.2
通讯作者：
Huang, Keng
中科院分区：
文献类型：
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作者：
Lin, Chang-min;Yuan, Yan-ping;Huang, Keng
大鼠须毛毛囊（HF）是研究HF或毛乳头（DP）重建的模型，其中Wnt/β-catenin信号通路是HF发生和出生后HF循环的关键通路。研究表明，Wnt/catenin信号通路在人或大鼠皮毛发育和出生后生长中起着不可或缺的作用。然而，一些Wnt/β-catenin信号通路因子的分布及其与胡须毛囊上皮干细胞标志物的关系尚未得到表征。在这项研究中，我们研究了Wnt/catenin信号通路成员的免疫定位，包括Wnt 10 b，Wnt 10a，Wnt 5a，β-catenin，和下游淋巴增强子结合因子1（LEF 1）和转录因子3（TCF 3），以及HF干细胞标志物CD 34，CK 15和增殖细胞核抗原（PCNA）蛋白，在大鼠生长期须状卵泡。β-连环蛋白、Wnt 5a、Wnt 10 b、Wnt 10a、LEF 1和TCF 3在生长期毛囊的外根鞘（ORS）、内根鞘、基质和毛干中表达。β-连环蛋白、Wnt 10 b、LEF 1和TCF 3在DP和真皮鞘（DS）区域中高度表达，而Wnt 5a和Wnt 10a在DP和真皮鞘（DS）区域中弱表达。α-平滑肌肌动蛋白的表达在较低的DS中是强的，并且在一些DP细胞中也检测到。CD 34、CK 15和PCNA在ORS中均表达，在基质中也有表达，但CD 34主要表达于DP和DS区。我们的研究定位了Wnt信号通路、下游LEF 1和TCF 3以及干细胞标记蛋白的位置，为理解Wnt信号通路在胡须毛囊生长中的作用提供了新的信息。
The rat whisker hair follicle (HF) is a model for studying the reconstruction of the HF or dermal papilla (DP), and involves the Wnt/beta-catenin signaling pathway, which is a key pathway in HF development and HF cycling after birth. It has been reported that Wnt/catenin signaling plays an indispensable role in human or rat pelages development and postnatal growth. However, the distribution of some Wnt/beta-catenin signaling pathway factors and their relationship with the epithelial stem cell markers in whisker follicles has not been characterized. In this study, we investigated the immunolocalization of Wnt/catenin signaling pathway members, including Wnt10b, Wnt10a, Wnt5a, beta-catenin, and downstream lymphoid enhancer-binding factor 1 (LEF1) and transcription factor 3 (TCF3), as well as, HF stem-cell markers CD34, CK15 and proliferating cell nuclear antigen (PCNA) protein, in rat anagen phase whisker follicles. beta-catenin, Wnt5a, Wnt10b, Wnt10a, LEF1, and TCF3 were expressed in the outer root sheath (ORS), inner root sheath, matrix and hair shaft of anagen follicles. beta-catenin, Wnt10b, LEF1, and TCF3 were highly expressed and Wnt5a and Wnt10a weakly expressed in DP and dermal sheath (DS) regions. The expression of alpha-smooth muscle actin was strong in the lower DS and it was also detected in some DP cells. CD34, CK15 and PCNA were all expressed in the ORS; and CD34 and PCNA were also detected in the matrix, however CD34 was extensively expressed in DP and DS regions. Our studies located the position of Wnts, downstream LEF1 and TCF3 and stem cell marker proteins, which provide new information in understanding the role of the Wnt singaling pathway in whisker follicles' growth.