Budding Yeast for Budding Geneticists: A Primer on the Saccharomyces cerevisiae Model System
Budding Yeast for Budding Geneticists: A Primer on the Saccharomyces cerevisiae Model System
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DOI：
10.1534/genetics.114.163188
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发表时间：
2014-05-01
期刊：
影响因子：
3.3
通讯作者：
Keeney, Jill B.
中科院分区：
文献类型：
--
作者：
Duina, Andrea A.;Miller, Mary E.;Keeney, Jill B.
出芽酵母酿酒酵母是研究真核细胞生物学基本方面的一种强大模式生物。这篇入门文章简要回顾了过去一个世纪中这种生物作为首要实验系统出现的历史。还概述了酿酒酵母基因组的核心特征，包括其遗传元件的性质和总体组织。以一种旨在吸引和挑战渴望更多了解出芽酵母实验适应性的本科生和研究生的方式，介绍了酵母遗传学家可用的一些最常见的实验工具和资源。最后，对源自酵母研究的几项重大发现的讨论凸显了酵母系统已经并将继续对我们理解与所有真核生物（包括人类）相关的各种细胞过程产生的深远影响。
The budding yeast Saccharomyces cerevisiae is a powerful model organism for studying fundamental aspects of eukaryotic cell biology. This Primer article presents a brief historical perspective on the emergence of this organism as a premier experimental system over the course of the past century. An overview of the central features of the S. cerevisiae genome, including the nature of its genetic elements and general organization, is also provided. Some of the most common experimental tools and resources available to yeast geneticists are presented in a way designed to engage and challenge undergraduate and graduate students eager to learn more about the experimental amenability of budding yeast. Finally, a discussion of several major discoveries derived from yeast studies highlights the far-reaching impact that the yeast system has had and will continue to have on our understanding of a variety of cellular processes relevant to all eukaryotes, including humans.