Cu@Au alloy nanoparticle as oligonucleotides labels for electrochemical stripping detection of DNA hybridization
Cu@Au alloy nanoparticle as oligonucleotides labels for electrochemical stripping detection of DNA hybridization
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DOI：
10.1016/s0956-5663(03)00084-8
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发表时间：
2003-10-01
影响因子：
12.6
通讯作者：
Fang, YZ
中科院分区：
文献类型：
--
作者：
Cai, H;Zhu, NN;Fang, YZ
本文介绍了新型Cu@Au合金纳米粒子的合成及其在电化学DNA杂交检测中的应用。我们报告了一种低温方法，用于生成由Cu的核和Au壳的薄层组成的核-壳颗粒，其可以容易地用寡核苷酸官能化。核-壳Cu@Au颗粒被成功地标记到与大肠杆菌毒素基因相关的5 ′-烷乙氧基封端的寡核苷酸探针上。DNA基因传感技术是利用聚吡咯（PPy）在玻碳电极（GCE）上的静电吸附作用，使目标寡核苷酸与聚吡咯（PPy）合金粒子-寡核苷酸DNA探针杂交而实现的。通过金属氧化溶解释放锚定在杂交体上的铜金属原子和通过灵敏的阳极溶出伏安法（ASV）间接测定溶解的Cu 2+离子来监测探针和靶之间的杂交事件。检测限为5.0 pmol l（-1）的靶寡核苷酸。Cu@Au核壳纳米粒子结合了Au的表面修饰特性和Cu核良好的电化学活性，在电化学DNA杂交分析中具有良好的应用前景。(C)2003 Elsevier Science B. V.保留所有权利。
Synthesis of the novel Cu@Au alloy nanoparticle and its application in an electrochemical DNA hybridization detection assay is described in this article. We report a low-temperature method for generating core-shell particles consisting of a core of Cu and a thin layer of Au shell that can be readily functionalized with oligonucleotides. Core-shell Cu@Au particles were successfully labeled to a 5'-alkanethiol capped oligonucleotides probe that is related to the colitoxin gene. The DNA genetic sensing assay relies oil the electrostatic adsorption of target oligonucleotides onto conducting polypyrrole (PPy) surface at the glassy carbon electrode (GCE), and its hybridization to the alloy particle-oligonucleotides DNA probe. Hybridization events between probe and target were monitored by the release of the copper metal atoms anchored on the hybrids by oxidative metal dissolution and the indirectly determination of the solubilized Cu2+ ions by sensitive anodic stripping voltammetry (ASV). The detection limit is 5.0 pmol l(-1) of target oligonucleotides. The Cu@Au core-shell nanoparticles combining the surface modification properties of Au with the good electrochemical activity of Cu core shows their perspective application in the electrochemical DNA hybridization analysis assay. (C) 2003 Elsevier Science B.V. All rights reserved.