Alleles Affecting 30 Traits for Productivity in Two Japonica Rice Varieties, Koshihikari and Nipponbare (Oryza sativa L.)
Alleles Affecting 30 Traits for Productivity in Two Japonica Rice Varieties, Koshihikari and Nipponbare (Oryza sativa L.)
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DOI：
10.1626/pps.17.47
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发表时间：
2014-01
影响因子：
2.5
通讯作者：
K. Ujiie;K. Ishimaru
中科院分区：
文献类型：
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作者：
K. Ujiie;K. Ishimaru
摘要利用2套染色体片段代换系（CSSLs）对30个与产量相关的性状进行了染色体区域影响性状（CRATs）的鉴定。一个是以日本晴为供体，以越光为背景品种（Kos-Nip），另一个是以越光为供体，以日本晴为背景品种（Nip-Kos）。在Kos-Nip和Nip-Kos CSSLs中，我们分别为30个性状确定了249个和181个CRATs。供体等位基因在75（日本晴）和82（越光）CRAT生产力有积极的影响。其中，一些CRATs表现出比籼稻品种Kasalath和Nona Bokra的等位基因更优越的上级效应。在1号染色体上，与越光相比，穗数（PN 1）的CRAT增加了约1.4倍的产量。PN 1增加了叶面积，同时保持了SPAD值，这是光合能力的指标。因此，PN 1可能对库容量和源能力具有多效性，从而提高产量。在13个性状的29个CRATs（占总数的12%）中，两套CSSLs都检测到了具有相反效应的CRATs。这些结果表明，基因的效应可能受到遗传背景的影响。通过使用Koshihikari和日本晴的单核苷酸多态性数据库，我们可以将28/29个CRAT的候选基因缩小到至少6个CRAT基因，这些基因与早期株高和茎粗相关。因此，粳稻可以作为一个有用的遗传供体，以提高另一个粳稻品种的生产力。
Abstract We identified chromosome regions affecting traits (CRATs) for 30 productivity-related traits using 2 sets of chromosome segment substitution lines (CSSLs). One was established using Nipponbare as the donor and Koshihikari as the background variety (Kos-Nip); and the other using Koshihikari as the donor and Nipponbare as the background (Nip-Kos). We identified 249 and 181 CRATs for 30 traits in Kos-Nip and Nip-Kos CSSLs, respectively. Donor alleles in 75 (Nipponbare) and 82 (Koshihikari) CRATs had positive effects on productivity. Among them, some CRATs represented superior effects as compared with the alleles in indica varieties Kasalath and Nona Bokra. On chromosome 1, a CRAT for panicle number (PN1) increased yield by about 1.4 times compared with Koshihikari. PN1 increased leaf area, while maintaining the SPAD value, which is an indicator of photosynthetic ability. Therefore, PN1 might have a pleiotropic effect on sink size and source ability, and thus could consequently improve yield. In only 29 CRATs for 13 traits (12%of total), corresponding CRATs with contrasting effects were detected in the 2 sets of CSSLs. These results suggested that the effect of a gene, might be affected by the genetic background. By using a database of single-nucleotide polymorphisms in Koshihikari and Nipponbare, we could narrow the candidate genes for 28/29 CRATs; to minimum 6 genes for CRAT related to plant height at the early stage and diameter at stem. Thus japonica rice could be a useful genetic donor for improving the productivity of another japonica variety.