Evidence for widespread Wolbachia infection in isopod crustaceans:: molecular identification and host feminization
Evidence for widespread Wolbachia infection in isopod crustaceans:: molecular identification and host feminization
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DOI：
10.1098/rspb.1998.0402
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发表时间：
1998-06-22
影响因子：
4.7
通讯作者：
Juchault, P
中科院分区：
文献类型：
--
作者：
Bouchon, D;Rigaud, T;Juchault, P
沃尔巴克氏菌是一种母系遗传的细胞内阿尔法变形杆菌，可感染多种节肢动物。它们在寄主中引起三种生殖变化：细胞质不亲和、孤雌生殖和雌性化。关于沃尔巴克氏菌在节肢动物中的分布已有许多研究，但已知的甲壳类物种感染的很少。我们调查了五个甲壳纲85个物种的沃尔巴克氏菌的流行情况。22种等足类动物被发现携带这些细菌。这种细菌主要在陆地物种中发现，这表明沃尔巴克氏菌来自大陆环境。通过细菌基因测序和种间转移来确定这些沃尔巴克氏菌菌株之间的进化关系。根据16S rDNA序列数据，所有与等足类相关的细菌都属于沃尔巴克氏B类。除1个外，该类群中的所有陆地等足类共生体都形成了一个独立的支系。种间转移的结果表明，沃尔巴克氏共生体对其等足目宿主具有专化性。他们还表明，在确定沃尔巴克氏菌感染的表型方面，寄主物种比细菌系统发育起着更重要的作用。
Wolbachia are maternally inherited, intracellular, alpha proteobacteria that infect a wide range of arthropods. They cause three kinds of reproductive alterations in their hosts: cytoplasmic incompatibility, parthenogenesis and feminization. There have been many studies of the distribution of Wolbachia in arthropods, but very few crustacean species are known to be infected. We investigated the prevalence of Wolbachia in 85 species from five crustacean orders. Twenty-two isopod species were found to carry these bacteria. The bacteria were found mainly in terrestrial species, suggesting that Wolbachia came from a continental environment. The evolutionary relationships between these Wolbachia strains were determined by sequencing bacterial genes and by interspecific transfers. All the bacteria associated with isopods belonged to the Wolbachiae B group, based on 16S rDNA sequence data. All the terrestrial isopod symbionts in this group except one formed an independent clade. The results of interspecific transfers show evidence of specialization of Wolbachia symbionts to their isopod hosts. They also suggest that host species plays a more important role than bacterial phylogeny in determining the phenotype induced by Wolbachia infection.