Transcriptome analysis of interactions between silkworm and cytoplasmic polyhedrosis virus.
Transcriptome analysis of interactions between silkworm and cytoplasmic polyhedrosis virus.
复制标题
家蚕与胞质多角体病毒相互作用的转录组分析
DOI：
10.1038/srep24894
复制
发表时间：
2016-04-27
影响因子：
4.6
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Jiang L;Peng Z;Guo Y;Cheng T;Guo H;Sun Q;Huang C;Zhao P;Xia Q
家蚕桑质多角体病毒（BmCPV）特异性感染家蚕中肠（MG），增殖主要发生在后中肠（PM）。在本研究中，在 BmCPV 感染后 0、3、24 和 72 小时提取 MG 和脂肪体 (FB)。每个样本的总序列读数超过1510000，映射率超过95.3%。感染过程中 MG 转录本上调增加。基因本体 (GO) 类别显示，FB 中的抗氧化剂均上调，但 MG 中则没有。 BGI001299、BGI014434、BGI012068和BGI009201是具有跨膜转运功能的MG特异性基因，其表达由BmCPV诱导。 BGI001299、BGI014434 和 BGI012068 在整个 MG 中表达，可能参与 BmCPV 入侵。 BGI009201 仅在 PM 中表达，可能是 BmCPV 增殖所必需的。 BmPGRP-S2 和 BGI012452（一种推定的丝氨酸蛋白酶）由 BmCPV 诱导，可能参与针对 BmCPV 的免疫防御。 MG 72h时BmCPV S1、S2、S3、S6、S7表达量较高，S9无表达，表明结构蛋白编码基因表达时间较早。这些结果为了解 BmCPV 感染和宿主防御机制提供了见解。
Bombyx moricytoplasmic polyhedrosis virus (BmCPV) specifically infects silkworm midgut (MG) and multiplication occurs mainly in posterior midgut (PM). In this study, MG and fat body (FB) were extracted at 0, 3, 24, and 72 h after BmCPV infection. The total sequence reads of each sample were more than 1510000, and the mapping ratio exceeded 95.3%. Upregulated transcripts increased in MG during the infection process. Gene ontology (GO) categories showed that antioxidants were all upregulated in FB but not in MG. BGI001299, BGI014434, BGI012068, and BGI009201 were MG-specific genes with transmembrane transport function, the expression of which were induced by BmCPV. BGI001299, BGI014434, and BGI012068 expressed in entire MG and may be involved in BmCPV invasion. BGI009201 expressed only in PM and may be necessary for BmCPV proliferation. BmPGRP-S2 and BGI012452 (a putative serine protease) were induced by BmCPV and may be involved in immune defense against BmCPV. The expression level of BmCPV S1, S2, S3, S6, and S7 was high and there was no expression of S9 in MG 72 h, implying that the expression time of structural protein coding genes is earlier. These results provide insights into the mechanism of BmCPV infection and host defense.