Action of influenza virus neuraminidase on gangliosides
Action of influenza virus neuraminidase on gangliosides
复制标题
流感病毒神经氨酸酶对神经节苷脂的作用
DOI：
10.1016/0014-5793(85)80314-8
复制
发表时间：
1985
期刊：
影响因子：
3.5
通讯作者：
L. Bergelson
中科院分区：
文献类型：
--
作者：
V. Slepushkin;A. Bukrinskaya;N. V. Prokazova;L.S. Zhigis;P.D. Reshetov;J.I. Shaposhnikova;L. Bergelson
本文研究了甲型流感病毒部分纯化的神经氨酸酶（NA）、洗涤液溶解NA与血凝素（HA）的混合物以及完整病毒粒子对神经节苷脂GTlb、gdla、GDlb、gml的作用。病毒NA主要将gtlb转化为gdlb，仅形成少量GMl。发现HA能抑制病毒NA的水解活性。同时，病毒NA将gdla2定量转化为gml， gml不被酶水解。这些结果表明，NA的功能是将“初级”受体（如GTlb）转移到适当的碳水化合物序列（GDlb - like）中，这被认为是流感病毒接受所需的最小结构。
The action of partly purified neuraminidase (NA) of influenza A virus, a mixture of detergent solubilized NA and haemagglutinin (HA) and of intact virions on gangliosides GTlb, gdla, GDlb, gmlwas studied. The viral NA transformed gtlbmainly into GDlbWith formation of only minor amounts of GMl. HA was found to inhibit the hydrolysis activity of viral NA. At the same time viral NA transformed GDlaquantitatively into gmlwhich was not hydrolyzed by the enzyme. These results suggest that the function of NA is to transfer the ‘primary’ receptor (such as GTlb) into the proper carbohydrate sequence (GDlb‐like) which is proposed to serve as the minimal structure required for influenza virus reception.