The role of nanoparticle size in hemocompatibility
The role of nanoparticle size in hemocompatibility
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DOI：
10.1016/j.tox.2009.01.015
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发表时间：
2009-04-28
期刊：
影响因子：
4.5
通讯作者：
Froehlich, Eleonore
中科院分区：
文献类型：
--
作者：
Mayer, Andrea;Vadon, Maria;Froehlich, Eleonore
预计纳米颗粒物质将越来越多地用于工业和医疗应用。由于已知纳米颗粒由于其体积小而表现出独特和潜在的危险特性，因此毒性研究，风险评估和风险管理非常有趣。我们关注的是对人体血液的不良影响。需要特别注意的过程是凝血、引发炎症和免疫反应的反应以及溶血。从确定不同介质中的大小和表面电荷开始，我们评估了大小和表面电荷对诱导凝血、血小板活化、补体活化、粒细胞活化和溶血的影响。我们使用聚苯乙烯颗粒作为模型，因为它们有不同的大小，但表面电荷恒定。分散溶液中盐和蛋白质的存在增加了颗粒大小并中和了表面电荷。带正电的粒子在缓冲溶液中形成聚集体。确定了颗粒与基于荧光相关细胞分选的测定的干扰。正表面电荷诱导补体活化。小尺寸引起血栓细胞和粒细胞活化和溶血。在用于实验的溶液中，粒子大小和表面电荷的表征对于解释结果显得很重要。人体血液中不良反应的大小依赖性不是线性的；带负电的颗粒大于60纳米的流体力学直径似乎比小的血液毒性要小得多。2009爱思唯尔爱尔兰有限公司版权所有。
It is expected that nanoparticular matters will be increasingly used for industrial and medical applications. Since it is known that nanoparticles exhibit unique and potential hazardous properties due to their small size, toxicity studies, risk assessment and risk management are of great interest. We focussed on adverse effects on human blood. Processes which warrant special attention are clotting, reactions triggering inflammatory and immune responses and hemolysis. Starting with the determination of size and surface charge in different media we assessed the effect of size and surface charge on induction of coagulation, thrombocyte activation, complement activation, granulocyte activation and hemolysis. We used polystyrene particles as model because they are available in different sizes but constant surface charges. The presence of salts and of protein in the dispersion solution increased particle size and neutralized surface charge. Positively charged particles formed aggregates in buffered solution. Interference of the particles with assays based on fluorescence associated cell sorting was identified. Positive surface charge induced activation of complement. Small size caused thrombocyte and granulocyte activation, and hemolysis. A characterization of particle size and surface charge in the solutions used for the experiments appears important for interpretation of the results. The size dependency of adverse effects in human blood is not linear; negatively charged particles larger than 60 nm hydrodynamic diameter appear to be considerably less hematotoxic than smaller ones. (c) 2009 Elsevier Ireland Ltd. All rights reserved.