Microarray Analysis of Spaceflown Murine Thymus Tissue Reveals Changes in Gene Expression Regulating Stress and Glucocorticoid Receptors
Microarray Analysis of Spaceflown Murine Thymus Tissue Reveals Changes in Gene Expression Regulating Stress and Glucocorticoid Receptors
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太空飞行小鼠胸腺组织的芯片分析揭示了调控应激和糖皮质激素受体的基因表达变化
DOI：
10.1002/jcb.22547
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发表时间：
2010-05-15
影响因子：
4
通讯作者：
DeLuca, Dominick
中科院分区：
文献类型：
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作者：
Lebsack, Ty W.;Fa, Vuna;DeLuca, Dominick
航天飞行和模拟微重力对免疫系统的有害影响已被广泛记录在案。我们报道了8周龄的年轻成年C57BL/6NTac小鼠在航天飞机(STS-118)上暴露13天后，结合定量RT-PCR的微阵列基因表达分析。任务结束后，提取了太空飞行小鼠(Flt)以及年龄和性别匹配的地面对照小鼠的胸腺叶，这些小鼠同样被安置在动物围栏模块(AEM)中。提取信使核糖核酸，进行基因表达的自动阵列分析。对微阵列数据的检查显示，有970个单独的探针发生了1.5倍或更大的变化。当这些数据平均后(n=4)，我们发现了12个基因在航天飞行后显著上调或下调至少1.5倍(P
The detrimental effects of spaceflight and simulated microgravity on the immune system have been extensively documented. We report here microarray gene expression analysis, in concert with quantitative RT-PCR, in young adult C57BL/6NTac mice at 8 weeks of age after exposure to spaceflight aboard the space shuttle (STS-118) for a period of 13 days. Upon conclusion of the mission, thymus lobes were extracted from space flown mice (FLT) as well as age- and sex-matched ground control mice similarly housed in animal enclosure modules (AEM). mRNA was extracted and an automated array analysis for gene expression was performed. Examination of the microarray data revealed 970 individual probes that had a 1.5-fold or greater change. When these data were averaged (n = 4), we identified 12 genes that were significantly up- or down-regulated by at least 1.5-fold after spaceflight (P