Effect of ovarian hormones on genes promoting dendritic spines in laser-captured serotonin neurons from macaques
Effect of ovarian hormones on genes promoting dendritic spines in laser-captured serotonin neurons from macaques
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DOI：
10.1038/mp.2009.78
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发表时间：
2010-10-01
影响因子：
11
通讯作者：
Reddy, A. P.
中科院分区：
文献类型：
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作者：
Bethea, C. L.;Reddy, A. P.
树突棘是神经元可塑性的基本结构单位，促进树突棘重塑的级联作用集中在Rho GTP酶和肌动蛋白动力学下游效应因子上。在激素替代治疗的模型中，我们用互补DNA阵列分析了雌二醇(E)和孕酮(P)对激光捕捉猕猴5-羟色胺神经元这些级联基因表达的影响。去卵巢恒河猴安乐死前1个月接受安慰剂、E或E+P硅胶植入治疗。取中脑，切片，进行色氨酸羟化酶(TPH)免疫组织化学染色。用Arcturus激光解剖显微镜(PixCell II)激光捕获TPH阳性神经元。将激光捕获的5-羟色胺神经元(n=2只/治疗组)的RNA与恒河猴GeneChips杂交。经E+/-P处理后，744个探针组差异有统计学意义(P
Dendritic spines are the elementary structural units of neuronal plasticity and the cascades that promote dendritic spine remodeling center on Rho GTPases and downstream effectors of actin dynamics. In a model of hormone replacement therapy, we sought the effect of estradiol (E) and progesterone (P) on gene expression in these cascades in laser-captured serotonin neurons from rhesus macaques with complementary DNA array analysis. Ovariectomized rhesus macaques were treated with either placebo, E or E+P through Silastic implant for 1 month before euthanasia. The midbrain was obtained, sectioned and immunostained for tryptophan hydroxylase (TPH). TPH-positive neurons were laser captured using an Arcturus Laser Dissection Microscope (PixCell II). RNA from laser-captured serotonin neurons (n=2 animals/treatment) was hybridized to Rhesus Affymetrix GeneChips. With E+/-P treatment, there was a significant change in 744 probe sets (analysis of variance, P