Aromatization of androgens by human abdominal and breast fat tissue.
Aromatization of androgens by human abdominal and breast fat tissue.
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人类腹部和乳房脂肪组织对雄激素的芳香化。
DOI：
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发表时间：
1975
影响因子：
5.8
通讯作者：
K. Ryan
中科院分区：
文献类型：
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作者：
A. Nimrod;K. Ryan
通过在NADPH存在下与各种细胞制剂孵育标记底物来研究人类腹部、乳房和腋窝脂肪将雄激素转化为雌激素的能力。孵化产物经过酚类分配、纸层析、甲醚生成、重复层析和结晶，以冷载体参比标准恒定比活度。雄烯二酮被粗匀浆、碎屑、脱脂颗粒组分(1,000-100,000倍)和从腹部、乳房或腋下脂肪中分离的脂肪细胞转化为雌酮，并在较小程度上转化为17β-雌二醇。研究发现，睾酮和雄烯二酮一样具有芳香化活性，但在这种情况下，比雌酮生成更多的17β-雌二醇。以19-羟基雄烯二酮-2为底物，所得结果与雄烯二酮相似。从乳腺癌获得的脂肪组织被发现与正常乳房脂肪(1-4pg/g脂肪/90分钟)的活性相同，并在腹部脂肪的范围内(1-27pg/g脂肪/90分钟)。在每一种情况下，腋下脂肪与来自同一受试者的乳房脂肪进行比较，腋下脂肪的活性都要高出5到10倍。结果表明，脂肪组织可能是性腺外雌激素的重要来源。
The ability of human abdominal, breast and axillary fat to convert androgens into estrogens was investigated by incubating labeled substrates in the presence of NADPH with a variety of cell preparations. The incubation products were subjected to phenolic partition, paper chromatography, methyl-ether formation, repeat chromatography and crystallization with cold carrier reference standards to constant specific activity. Androstenedione was converted to estrone and, to a lesser extent, to 17beta-estradiol by crude homogenates, minces, fat-free particulate fractions (1,000-100,000 time g) and isolated fat cells obtained from abdominal, breast or axillary fat. Testosterone was found to be aromatized as actively as androstenedione, but inthis case more 17 beta-estrodiol was formed than estrone. 19-Hydroxyandrostenedione-2 also served as substrate, givingresults similar to those obtained with androstenedione. Fat tissue obtained from cancerous breasts was found to be as active as normal breast fat (1-4 pg/g fat/90 min) and within the range found for abdominal fat (1-27 pg/g fat/90 min). In each case in which axillary fat was compared to breast fat from the same subject, the activity of the axillary fat was 5 to 10 times higher. The results indicate a possible role of adipose tissue as a significant extra-gonadal source of estrogens.